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Protein Damage Caused by Occupational Toxicants.

PB98137771

Publication Date	1997
Page Count	12
Abstract	Exposure of certain classes of industrial toxicants has been shown to result in oxidative damage. Metal ions have been shown to cause oxidative injury, which may contribute to the role of metal-induced fibrosis and carcinogenesis. The authors have demonstrated that proteins are potential targets of metal-catalyzed oxidative damage. The authors developed polyclonal antibody techniques to detect oxidatively damaged proteins. The formation of carbonyl moieties has been demonstrated to result from oxidative damage to proteins, such as occurs from exposure to oxidants in industrial environments. The techniques the authors developed involved reaction of the carbonyl moieties derived from oxidized proteins with 2,4-dinitrophenylhydrazine and subsequent detection with an antibody. Proteins, both purified and from fractions of mouse tissue, were oxidized in vitro using several sources of oxidants, including metal-ions of occupational importance.
Keywords	Toxic substances Protein damage Occupational safety and health Oxidation Metal ions Fibrosis Carcinogenesis Carbonyl mioeties Polyclonal anibody techniques
Source Agency	Department H.E.W. Office of Toxic Substance and Disease
Corporate Authors	Agency for Toxic Substances and Disease Registry, Atlanta, GA.
Document Type	Technical Report
Title Note	Final performance rept.
NTIS Issue Number	199813

Protein Damage Caused by Occupational Toxicants.

Protein Damage Caused by Occupational Toxicants.
PB98137771

Publication Date	1997
Page Count	12
Abstract	Exposure of certain classes of industrial toxicants has been shown to result in oxidative damage. Metal ions have been shown to cause oxidative injury, which may contribute to the role of metal-induced fibrosis and carcinogenesis. The authors have demonstrated that proteins are potential targets of metal-catalyzed oxidative damage. The authors developed polyclonal antibody techniques to detect oxidatively damaged proteins. The formation of carbonyl moieties has been demonstrated to result from oxidative damage to proteins, such as occurs from exposure to oxidants in industrial environments. The techniques the authors developed involved reaction of the carbonyl moieties derived from oxidized proteins with 2,4-dinitrophenylhydrazine and subsequent detection with an antibody. Proteins, both purified and from fractions of mouse tissue, were oxidized in vitro using several sources of oxidants, including metal-ions of occupational importance.
Keywords	Toxic substances Protein damage Occupational safety and health Oxidation Metal ions Fibrosis Carcinogenesis Carbonyl mioeties Polyclonal anibody techniques
Source Agency	Department H.E.W. Office of Toxic Substance and Disease
Corporate Authors	Agency for Toxic Substances and Disease Registry, Atlanta, GA.
Document Type	Technical Report
Title Note	Final performance rept.
NTIS Issue Number	199813