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Grain Dust Induced Lung Inflammation Is Reduced by 'Rhodobacter sphaeroides' Diphosphoryl Lipid A.

PB97207138

Publication Date	1996
Personal Author	Jagielo, P. J.; Quinn, T. J.; Qureshi, N.; Schwartz, D. A.
Page Count	28
Abstract	The efficacy of pentaacylated diphosphoryl-lipid-A derived from the lipopolysaccharide (LPS) of Rhodobacter-spheroides (RsDPLA) was examined as a competitive inhibitor of grain dust induced airway inflammation. TTHP-1 cells were incubated with RsDPLA, LPS, or cotton dust extract (CDE). Incubation with LPS or CDE resulted in TNF-alpha release at 0.02 microgram/milliliter. Pretreatment of THP-1 cells with varying concentrations of RsDPLA prior to incubation with LPS or CDE resulted ina dose dependent reduction in the LPS or CDE induced tumor necrosis factor alpha (TNF-alpha) release. The unique LPS inhibitory property of RsDPLA was used to determine the inflammatory response to inhaled CDE in mice in the presence of RsDPLA. No significant inflammatory response was caused by 10 micrograms of RsDPLA intratracheally in comparison to intratracheal saline. Pretreatment with RsDPLA intratracheally prior to exposure to CDE or LPS resulted in significant reductions in the lung lavage concentrations of total cells, neutrophils, TNF-alpha, and interleukin-6 compared to mice pretreated with sterile saline. The authors conclude the LPS inhibitory effect of RsDPLA is confirmed.
Keywords	Pulmonary system disorders Lung irritants Dust exposure Organic dusts Inhalants Grain dusts In vitro analysis In vivo analysis Laboratory animals Inflammation Lipopolysaccharides Rhodobacter-spheroides
Source Agency	National Institute for Occupational Safety and Health
Corporate Authors	Iowa Univ., Iowa City. Coll. of Medicine.; Wisconsin Univ.-Madison. Dept. of Bacteriology.; National Inst. for Occupational Safety and Health, Cincinnati, OH.; Department of Veterans Affairs, Washington, DC. Medical Research Service.; William S. Middleton Memorial Veteran's Hospital, Madison, WI.; National Inst. of Environmental Health Sciences, Research Triangle Park,
Supplemental Notes	Prepared in cooperation with Wisconsin Univ.-Madison. Dept. of Bacteriology. and William S. Middleton Memorial Veteran's Hospital, Madison, WI. Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH., National Inst. of Environmental Health Sciences, Research Triangle Park, NC. and Department of Veterans Affairs, Washington, DC. Medical Research Service.
Document Type	Technical Report
NTIS Issue Number	199724

Grain Dust Induced Lung Inflammation Is Reduced by 'Rhodobacter sphaeroides' Diphosphoryl Lipid A.

Grain Dust Induced Lung Inflammation Is Reduced by 'Rhodobacter sphaeroides' Diphosphoryl Lipid A.
PB97207138

Publication Date	1996
Personal Author	Jagielo, P. J.; Quinn, T. J.; Qureshi, N.; Schwartz, D. A.
Page Count	28
Abstract	The efficacy of pentaacylated diphosphoryl-lipid-A derived from the lipopolysaccharide (LPS) of Rhodobacter-spheroides (RsDPLA) was examined as a competitive inhibitor of grain dust induced airway inflammation. TTHP-1 cells were incubated with RsDPLA, LPS, or cotton dust extract (CDE). Incubation with LPS or CDE resulted in TNF-alpha release at 0.02 microgram/milliliter. Pretreatment of THP-1 cells with varying concentrations of RsDPLA prior to incubation with LPS or CDE resulted ina dose dependent reduction in the LPS or CDE induced tumor necrosis factor alpha (TNF-alpha) release. The unique LPS inhibitory property of RsDPLA was used to determine the inflammatory response to inhaled CDE in mice in the presence of RsDPLA. No significant inflammatory response was caused by 10 micrograms of RsDPLA intratracheally in comparison to intratracheal saline. Pretreatment with RsDPLA intratracheally prior to exposure to CDE or LPS resulted in significant reductions in the lung lavage concentrations of total cells, neutrophils, TNF-alpha, and interleukin-6 compared to mice pretreated with sterile saline. The authors conclude the LPS inhibitory effect of RsDPLA is confirmed.
Keywords	Pulmonary system disorders Lung irritants Dust exposure Organic dusts Inhalants Grain dusts In vitro analysis In vivo analysis Laboratory animals Inflammation Lipopolysaccharides Rhodobacter-spheroides
Source Agency	National Institute for Occupational Safety and Health
Corporate Authors	Iowa Univ., Iowa City. Coll. of Medicine.; Wisconsin Univ.-Madison. Dept. of Bacteriology.; National Inst. for Occupational Safety and Health, Cincinnati, OH.; Department of Veterans Affairs, Washington, DC. Medical Research Service.; William S. Middleton Memorial Veteran's Hospital, Madison, WI.; National Inst. of Environmental Health Sciences, Research Triangle Park,
Supplemental Notes	Prepared in cooperation with Wisconsin Univ.-Madison. Dept. of Bacteriology. and William S. Middleton Memorial Veteran's Hospital, Madison, WI. Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH., National Inst. of Environmental Health Sciences, Research Triangle Park, NC. and Department of Veterans Affairs, Washington, DC. Medical Research Service.
Document Type	Technical Report
NTIS Issue Number	199724