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Expression and Activity of Urokinase and Its Receptor in Endothelial Cells Exposed to Asbestos.

PB97206304

Publication Date	1997
Personal Author	Treadwell, M. D.; Fava, R. A.; Hunt, J. A.; Krieser, R. J.; Barchowsky, A.
Page Count	48
Abstract	Endothelial cells were exposed to chrysotile (12001295), crocidolite (12001284), or refractory-ceramic-fiber-1 (RCF-1) in an effort to determine whether asbestos induced endothelial cell activiation is associated with altered proteolysis and expression of urokinase-like- plasminogen-activator (uPA) and urokinase-like-plasminogen- activator-receptor (uPAR). Second or third passage porcine aortice endothelial cells grown to the confluence were incubated with the fibers for up to 24 hours. In-situ zymography for uPAactivity in exposed cultures demonstrated that these fibers produce localized increases in proteolysis. Within 8 hours, exposure to chrysotile or crocidolite increased uPA epxression. The increases in proteolysis correlate well with increases in steady state uPA mRNA levels and with increased levels of uPAR protein. In contrast, rRCF-1 has a lower fibrogenic potential than asbestos and it failed elicit endothelial cell activation or affect uPA/uPAR. The authors suggest thatasbestos fibers directly activate vascular endothelial cells to express proteins that facilitate tissue remodeling.
Keywords	Lung irritants Pulmonary system disorders Occupational safety and health Asbestos fibers Fibrous dusts Cytotoxic effects Cell damage Mammalian cells Urokinase Endothelia cells
Source Agency	National Institute for Occupational Safety and Health
Corporate Authors	Dartmouth Medical School, Hanover, NH. Dept. of Pharmacology and Toxicology.; National Inst. for Occupational Safety and Health, Cincinnati, OH.; Veterans Hospital, White River Junction, VT. Dept. of Medicine.
Supplemental Notes	Prepared in cooperation with Veterans Hospital, White River Junction, VT. Dept. of Medicine. Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	199724

Expression and Activity of Urokinase and Its Receptor in Endothelial Cells Exposed to Asbestos.

Expression and Activity of Urokinase and Its Receptor in Endothelial Cells Exposed to Asbestos.
PB97206304

Publication Date	1997
Personal Author	Treadwell, M. D.; Fava, R. A.; Hunt, J. A.; Krieser, R. J.; Barchowsky, A.
Page Count	48
Abstract	Endothelial cells were exposed to chrysotile (12001295), crocidolite (12001284), or refractory-ceramic-fiber-1 (RCF-1) in an effort to determine whether asbestos induced endothelial cell activiation is associated with altered proteolysis and expression of urokinase-like- plasminogen-activator (uPA) and urokinase-like-plasminogen- activator-receptor (uPAR). Second or third passage porcine aortice endothelial cells grown to the confluence were incubated with the fibers for up to 24 hours. In-situ zymography for uPAactivity in exposed cultures demonstrated that these fibers produce localized increases in proteolysis. Within 8 hours, exposure to chrysotile or crocidolite increased uPA epxression. The increases in proteolysis correlate well with increases in steady state uPA mRNA levels and with increased levels of uPAR protein. In contrast, rRCF-1 has a lower fibrogenic potential than asbestos and it failed elicit endothelial cell activation or affect uPA/uPAR. The authors suggest thatasbestos fibers directly activate vascular endothelial cells to express proteins that facilitate tissue remodeling.
Keywords	Lung irritants Pulmonary system disorders Occupational safety and health Asbestos fibers Fibrous dusts Cytotoxic effects Cell damage Mammalian cells Urokinase Endothelia cells
Source Agency	National Institute for Occupational Safety and Health
Corporate Authors	Dartmouth Medical School, Hanover, NH. Dept. of Pharmacology and Toxicology.; National Inst. for Occupational Safety and Health, Cincinnati, OH.; Veterans Hospital, White River Junction, VT. Dept. of Medicine.
Supplemental Notes	Prepared in cooperation with Veterans Hospital, White River Junction, VT. Dept. of Medicine. Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	199724