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Asbestos Induced Alteration in Endothelial Cell Function.

PB97206296

Publication Date	1997
Personal Author	Treadwell, M. D.
Page Count	8
Abstract	The purpose of this research was to investigate whether asbestos (1332214) induces an active endothelial cell phenotype, resulting in theincreased expression of growth factors and proteases, which are relevantto the development of fibrosis. Porcine aortic endothelial cells were cultured with asbestos and ceramic fibers. The findings indicated that endothelial cells respond to noncytotoxic concentrations of asbestos fibers with alterations in specific cell functions, which may be relevant to pulmonary diseases processes. The cells respond to both chrysotile (12001293) and crocidolite (12001284) asbestos fibers by changing cell morphology in focal areas of fiber contact, assuming a spindled appearance, indicative of the activated endothelial cell. A time dependent increase was noted in neutrophil binding to Intercellular-Adhesion-Molecule-1 and increases in both Urokinase-like- Plasminogen-Activator (uPA) mRNA levels and uPA activity.Endothelial cells exposed to chrysotile asbestos demonstrated in a time dependent manner an increase in the expression of the cell surface uPA receptor.
Keywords	Pulmonary system disorders Asbestos fibers Occupational health Fibrous dusts Lung irritants Respiratory system disorders Cytotoxic effects Cell cultures Mammalian cells
Source Agency	National Institute for Occupational Safety and Health
Corporate Authors	Lockheed Sanders, Inc., Nashua, NH.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
Title Note	Final rept.
NTIS Issue Number	199723

Asbestos Induced Alteration in Endothelial Cell Function.

Asbestos Induced Alteration in Endothelial Cell Function.
PB97206296

Publication Date	1997
Personal Author	Treadwell, M. D.
Page Count	8
Abstract	The purpose of this research was to investigate whether asbestos (1332214) induces an active endothelial cell phenotype, resulting in theincreased expression of growth factors and proteases, which are relevantto the development of fibrosis. Porcine aortic endothelial cells were cultured with asbestos and ceramic fibers. The findings indicated that endothelial cells respond to noncytotoxic concentrations of asbestos fibers with alterations in specific cell functions, which may be relevant to pulmonary diseases processes. The cells respond to both chrysotile (12001293) and crocidolite (12001284) asbestos fibers by changing cell morphology in focal areas of fiber contact, assuming a spindled appearance, indicative of the activated endothelial cell. A time dependent increase was noted in neutrophil binding to Intercellular-Adhesion-Molecule-1 and increases in both Urokinase-like- Plasminogen-Activator (uPA) mRNA levels and uPA activity.Endothelial cells exposed to chrysotile asbestos demonstrated in a time dependent manner an increase in the expression of the cell surface uPA receptor.
Keywords	Pulmonary system disorders Asbestos fibers Occupational health Fibrous dusts Lung irritants Respiratory system disorders Cytotoxic effects Cell cultures Mammalian cells
Source Agency	National Institute for Occupational Safety and Health
Corporate Authors	Lockheed Sanders, Inc., Nashua, NH.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
Title Note	Final rept.
NTIS Issue Number	199723