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Cocarcinogenicity of Particles in Coculture System.

PB97105183

Publication Date	1995
Personal Author	Cheu, C. J.
Page Count	66
Abstract	The purpose of this study was to investigate the effects of particles on the metabolism of benzo(a)pyrene (BaP) and the formation of BaP DNA adducts. Hamster pulmonary alveolar macrophages (AM) were used because of their phagocytic, metabolic, secretory and migratory behavior. Cocultivation AM with hamster tracheal epithelial cells was based on the metabolic role of AM, mucociliary escalator clearance of particles and pathological epithelial origins of pulmonary carcinoma. Our results indicate that Fe2O3, not Al2O3, can enhance the metabolism of BaP in AM and subsequently, the formation of DNA adducts in tracheal epithelial cells can also be elevated. Both metabolism of BaP and DNA adduct formation can be diminished by addition of P-450s and epoxide hydrolase inhibitors. Two of the DNA adducts were partially characterized by co-migration of the standards of (-)anti-BPDE-dG and (+)-anti-BPDE-dG adducts.
Keywords	Carcinogenesis Particles Cells(Biology) Metabolism Hamsters Laboratory animals Macrophages Cell lines Coculture systems Benzo(a)pyrene DNA adducts
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57Y - Toxicology 57S - Physiology
Corporate Authors	Cincinnati Univ. Medical Center, OH. Dept. of Environmental Health.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Thesis
Title Note	Thesis.
NTIS Issue Number	199701

Cocarcinogenicity of Particles in Coculture System.

Cocarcinogenicity of Particles in Coculture System.
PB97105183

Publication Date	1995
Personal Author	Cheu, C. J.
Page Count	66
Abstract	The purpose of this study was to investigate the effects of particles on the metabolism of benzo(a)pyrene (BaP) and the formation of BaP DNA adducts. Hamster pulmonary alveolar macrophages (AM) were used because of their phagocytic, metabolic, secretory and migratory behavior. Cocultivation AM with hamster tracheal epithelial cells was based on the metabolic role of AM, mucociliary escalator clearance of particles and pathological epithelial origins of pulmonary carcinoma. Our results indicate that Fe2O3, not Al2O3, can enhance the metabolism of BaP in AM and subsequently, the formation of DNA adducts in tracheal epithelial cells can also be elevated. Both metabolism of BaP and DNA adduct formation can be diminished by addition of P-450s and epoxide hydrolase inhibitors. Two of the DNA adducts were partially characterized by co-migration of the standards of (-)anti-BPDE-dG and (+)-anti-BPDE-dG adducts.
Keywords	Carcinogenesis Particles Cells(Biology) Metabolism Hamsters Laboratory animals Macrophages Cell lines Coculture systems Benzo(a)pyrene DNA adducts
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57Y - Toxicology 57S - Physiology
Corporate Authors	Cincinnati Univ. Medical Center, OH. Dept. of Environmental Health.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Thesis
Title Note	Thesis.
NTIS Issue Number	199701