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Plasma Proteins: Markers of Chemical Exposure.

PB94177110

Publication Date	1993
Personal Author	Ansari, G. A. S.; Gan, J. C.
Page Count	7
Abstract	The use of plasma proteins as markers of chemical exposure was studied. The specific areas of investigation were the covalent binding of acrolein (107028) to albumin, the rapid quantitation of acrolein and crotonaldehyde (4170303) modified albumin, the susceptibility to degradation of covalently modified albumin, the development of an enzyme linked immunosorbent assay (ELISA) for styrene (100425), and the preparation and detection of styrene-oxide (96093) specific antibodies. The authors suggest that more tyrosine and/or tryptophan residues were exposed as a result of the unfolding of the protein through covalent modification of the lysyl and histidyl residues. The preparation of a styrene-oxide albumin conjugate, the generation of polyclonal antibodies in rabbits, and an ELISA procedure for the detection of antibodies were also discussed.
Keywords	Blood proteins Blood plasma Biochemistry Biological effects Cytotoxins Chemical analysis Bioindicators In vivo analysis In vitro analysis Assaying Acrolein Albumins Aldehydes Styrene ELISA(Enzyme linked immunosorbent assay) CAS 107028 Crotonaldehyde CAS 4170303 CAS 100425 CAS 96093 Styrene oxide
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57F - Cytology, Genetics, & Molecular Biology 57Y - Toxicology 57B - Biochemistry
Corporate Authors	Texas Univ. Medical Branch at Galveston. Dept. of Human Biological Chemistry and Genetics.; Occupational Safety and Health Administration, Washington, DC.
Supplemental Notes	Sponsored by Occupational Safety and Health Administration, Washington, DC.
Document Type	Technical Report
Title Note	Final performance rept. 1 Sep 89-31 Aug 93.
NTIS Issue Number	199418

Plasma Proteins: Markers of Chemical Exposure.

Plasma Proteins: Markers of Chemical Exposure.
PB94177110

Publication Date	1993
Personal Author	Ansari, G. A. S.; Gan, J. C.
Page Count	7
Abstract	The use of plasma proteins as markers of chemical exposure was studied. The specific areas of investigation were the covalent binding of acrolein (107028) to albumin, the rapid quantitation of acrolein and crotonaldehyde (4170303) modified albumin, the susceptibility to degradation of covalently modified albumin, the development of an enzyme linked immunosorbent assay (ELISA) for styrene (100425), and the preparation and detection of styrene-oxide (96093) specific antibodies. The authors suggest that more tyrosine and/or tryptophan residues were exposed as a result of the unfolding of the protein through covalent modification of the lysyl and histidyl residues. The preparation of a styrene-oxide albumin conjugate, the generation of polyclonal antibodies in rabbits, and an ELISA procedure for the detection of antibodies were also discussed.
Keywords	Blood proteins Blood plasma Biochemistry Biological effects Cytotoxins Chemical analysis Bioindicators In vivo analysis In vitro analysis Assaying Acrolein Albumins Aldehydes Styrene ELISA(Enzyme linked immunosorbent assay) CAS 107028 Crotonaldehyde CAS 4170303 CAS 100425 CAS 96093 Styrene oxide
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57F - Cytology, Genetics, & Molecular Biology 57Y - Toxicology 57B - Biochemistry
Corporate Authors	Texas Univ. Medical Branch at Galveston. Dept. of Human Biological Chemistry and Genetics.; Occupational Safety and Health Administration, Washington, DC.
Supplemental Notes	Sponsored by Occupational Safety and Health Administration, Washington, DC.
Document Type	Technical Report
Title Note	Final performance rept. 1 Sep 89-31 Aug 93.
NTIS Issue Number	199418