Publication Date |
1990 |
Personal Author |
Blake, R. C.; Revis, N. W.; Holdsworth, G. |
Page Count |
50 |
Abstract |
Thiobacillus ferrooxidans is a prominent member of a group of chemo-lithotrophic bacteria that bear principal responsibility for the formation of acid mine drainage. A prototype enzyme-linked immunosorbent assay (ELISA) for enumerating and qualifying T. ferrooxidans was assembled and characterized. The immunoassay protocol consisted of sequential incubations of the sample with (i) the primary antibody, (ii) the enzyme-labeled secondary antibody, and (iii) a chromogenic substrate specific for the enzyme lable. The necessary reagents comprised primary polyclonal rabbit antibodies directed against T. ferrooxidans ATCC 23270, alkaline phosphatase-copled goat anti-rabbit polyclonal antibodies, and phenolphrhalein monophosphate. The ELISA developed herein correctly identified whether iron-oxidizing bacteria were present in each of 4 samples supplied and analyzed by an independent laboratory. Sufficient preliminary data was obtained to warrant further research and development activities. |
Keywords |
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Source Agency |
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NTIS Subject Category |
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Corporate Authors |
Oak Ridge Research Inst., TN.; Bureau of Mines, Pittsburgh, PA. Pittsburgh Research Center. |
Supplemental Notes |
Sponsored by Bureau of Mines, Pittsburgh, PA. Pittsburgh Research Center. |
Document Type |
Technical Report |
Title Note |
Technical rept. (Final). |
NTIS Issue Number |
199110 |
Contract Number |
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