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Mechanisms of Cytoskeletal Injury by Heavy Metals.

PB90163742

Publication Date	1989
Personal Author	Perrino, B. A.
Page Count	8
Abstract	The major aim of the study was to determine whether metallothionein (MT) disassembly and inhibition of assembly caused by cadmium (7440439), mercury (7439976), and lead (7439921) in cultured 3T3 cells was due to binding to tubulin sulfhydryl groups or binding to and activation of CaM. Methylmercury was used as a model for adverse effects to MT due to tubulin sulfhydryl binding in the extracted cytoskeleton model system and during the polymerization of purified MT proteins in-vitro. The results indicated that, like calcium, the inhibitory effect of cadmium on the polymerization of purified MT proteins containing tubulin and microtubule associated proteins was enhanced by exogenously added CaM, suggesting that microtubule associated proteins were involved in mediating the sensitivity of MT to cadmium activated CaM. The findings that cadmium, like calcium, can support the binding of CaM to tubulin and MAPs in Western blots of purified MT proteins support the proposal that MAPs are important to the increased sensitivity of MT to cadmium activated CaM. The stimulation by cadmium or calcium/CaM dependent protein-kinase-II, resulting in MAP phosphorylation and MT disassembly and inhibition of assembly was not investigated.
Keywords	Toxicity Proteins Exposure Lead(Metal) In vitro analysis Cadmium Heavy metals Metallothionein Cytoskeleton Protein kinases Methylmercury compounds CAS 7440-43-9 CAS 7439-97-6 CAS 7439-92-1 Western blotting Calmodulin Horseradish peroxidase Microtubule-associated protein
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57Y - Toxicology 57F - Cytology, Genetics, & Molecular Biology
Corporate Authors	Boston Univ., MA. School of Medicine.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	199009

Mechanisms of Cytoskeletal Injury by Heavy Metals.

Mechanisms of Cytoskeletal Injury by Heavy Metals.
PB90163742

Publication Date	1989
Personal Author	Perrino, B. A.
Page Count	8
Abstract	The major aim of the study was to determine whether metallothionein (MT) disassembly and inhibition of assembly caused by cadmium (7440439), mercury (7439976), and lead (7439921) in cultured 3T3 cells was due to binding to tubulin sulfhydryl groups or binding to and activation of CaM. Methylmercury was used as a model for adverse effects to MT due to tubulin sulfhydryl binding in the extracted cytoskeleton model system and during the polymerization of purified MT proteins in-vitro. The results indicated that, like calcium, the inhibitory effect of cadmium on the polymerization of purified MT proteins containing tubulin and microtubule associated proteins was enhanced by exogenously added CaM, suggesting that microtubule associated proteins were involved in mediating the sensitivity of MT to cadmium activated CaM. The findings that cadmium, like calcium, can support the binding of CaM to tubulin and MAPs in Western blots of purified MT proteins support the proposal that MAPs are important to the increased sensitivity of MT to cadmium activated CaM. The stimulation by cadmium or calcium/CaM dependent protein-kinase-II, resulting in MAP phosphorylation and MT disassembly and inhibition of assembly was not investigated.
Keywords	Toxicity Proteins Exposure Lead(Metal) In vitro analysis Cadmium Heavy metals Metallothionein Cytoskeleton Protein kinases Methylmercury compounds CAS 7440-43-9 CAS 7439-97-6 CAS 7439-92-1 Western blotting Calmodulin Horseradish peroxidase Microtubule-associated protein
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57Y - Toxicology 57F - Cytology, Genetics, & Molecular Biology
Corporate Authors	Boston Univ., MA. School of Medicine.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	199009