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Improved Method for the Direct Determination of Cadmium in Biological Materials by Atomic Absorption Spectrophotometry.

PB90129438

Publication Date	1989
Personal Author	Blackstone, M.; Kaplan, P.; Richdale, N.
Page Count	18
Abstract	A method was presented for the determination of metals in mammalian tissues using an atomic absorption method. The procedure calls for digestion of the sample with a quaternary ammonium-hydroxide. With the method one can measure metals which are normally present in low concentrations such as cadmium (7440439) and nickel (7440020) with significant sensitivity and a minimal amount of sample handling. Two month old rats were exposed through inhalation to cadmium-oxide (1306190) aerosols daily for 8 hour periods. The precision of the method was determined in repeated analyses of control and exposed lung tissue. All lungs were divided into approximately five equal portions and each portion analyzed for cadmium, nickel, and zinc (7440666). To determine cadmium and nickel concentrations, 200 to 300 milligrams of wet tissue are needed for precision. These weights can be reduced to 1/20 for the analysis of zinc and copper (7440508). The absorbed amounts were small, suggesting that if the cadmium-oxide aerosol was absorbed, it was also effectively cleared from the system.
Keywords	Cadmium Determination Rats Respiration Lung Metals Zinc Nickel Tables(Data) Atomic absorption spectrophotometry CAS 7440-50-8 Tissue distribution CAS 7440-43-9 CAS 7440-02-0 CAS 1306-19-0 CAS 7440-66-6
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57B - Biochemistry 57D - Clinical Chemistry 57E - Clinical Medicine
Corporate Authors	Cincinnati Univ., OH. Kettering Lab.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	199005

Improved Method for the Direct Determination of Cadmium in Biological Materials by Atomic Absorption Spectrophotometry.

Improved Method for the Direct Determination of Cadmium in Biological Materials by Atomic Absorption Spectrophotometry.
PB90129438

Publication Date	1989
Personal Author	Blackstone, M.; Kaplan, P.; Richdale, N.
Page Count	18
Abstract	A method was presented for the determination of metals in mammalian tissues using an atomic absorption method. The procedure calls for digestion of the sample with a quaternary ammonium-hydroxide. With the method one can measure metals which are normally present in low concentrations such as cadmium (7440439) and nickel (7440020) with significant sensitivity and a minimal amount of sample handling. Two month old rats were exposed through inhalation to cadmium-oxide (1306190) aerosols daily for 8 hour periods. The precision of the method was determined in repeated analyses of control and exposed lung tissue. All lungs were divided into approximately five equal portions and each portion analyzed for cadmium, nickel, and zinc (7440666). To determine cadmium and nickel concentrations, 200 to 300 milligrams of wet tissue are needed for precision. These weights can be reduced to 1/20 for the analysis of zinc and copper (7440508). The absorbed amounts were small, suggesting that if the cadmium-oxide aerosol was absorbed, it was also effectively cleared from the system.
Keywords	Cadmium Determination Rats Respiration Lung Metals Zinc Nickel Tables(Data) Atomic absorption spectrophotometry CAS 7440-50-8 Tissue distribution CAS 7440-43-9 CAS 7440-02-0 CAS 1306-19-0 CAS 7440-66-6
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57B - Biochemistry 57D - Clinical Chemistry 57E - Clinical Medicine
Corporate Authors	Cincinnati Univ., OH. Kettering Lab.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	199005