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Seed Germination and Root Elongation Toxicity Tests in Hazardous Waste Site Evaluation: Methods Development and Applications.

PB90113184

Publication Date	1989
Personal Author	Linder, G.; Greene, J. C.; Ratsch, H.; Nwosu, J.; Smith, S.
Page Count	34
Abstract	Seed germination tests measure soil toxicity directly, while root elongation tests consider the indirect effects of water-soluble constituents which may be present in site-samples. In the seed germination toxicity test, site-soil is mixed with a reference soil to yield exposure concentrations into which test seeds are planted. Germination is evaluated after a five-day exposure, and effective concentrations associated with a 50% reduction in seed germination are calculated. Root elongation tests evaluate site-soil eluates. Here seeds are placed onto moistened filter paper which lines Petri dish exposure chambers; exposure chambers are then covered and incubated in complete darkness for five days. Inhibition of root elongation is calculated as an EC50 (exposure concentration which yields a 50% reduction in root length relative to controls) upon termination of the test.
Keywords	Toxicity Germination Plants(Botany) Hazardous materials Waste disposal Mutagenicity tests Soil pollutants Superfund program Environmental monitoring
Source Agency	Environmental Protection Agency Office of Research and Development
NTIS Subject Category	68C - Solid Wastes Pollution & Control 57Y - Toxicology 57F - Cytology, Genetics, & Molecular Biology
Corporate Authors	Corvallis Environmental Research Lab., OR.; NSI Technology Services Corp., Corvallis, OR.
Supplemental Notes	Presented at Symposium on Use of Plants in Toxicity Assessment (1st), Atlanta, GA., April 16-21, 1989. Prepared in cooperation with NSI Technology Services Corp., Corvallis, OR.
Document Type	Technical Report
Title Note	Symposium paper.
NTIS Issue Number	199002

Seed Germination and Root Elongation Toxicity Tests in Hazardous Waste Site Evaluation: Methods Development and Applications.

Seed Germination and Root Elongation Toxicity Tests in Hazardous Waste Site Evaluation: Methods Development and Applications.
PB90113184

Publication Date	1989
Personal Author	Linder, G.; Greene, J. C.; Ratsch, H.; Nwosu, J.; Smith, S.
Page Count	34
Abstract	Seed germination tests measure soil toxicity directly, while root elongation tests consider the indirect effects of water-soluble constituents which may be present in site-samples. In the seed germination toxicity test, site-soil is mixed with a reference soil to yield exposure concentrations into which test seeds are planted. Germination is evaluated after a five-day exposure, and effective concentrations associated with a 50% reduction in seed germination are calculated. Root elongation tests evaluate site-soil eluates. Here seeds are placed onto moistened filter paper which lines Petri dish exposure chambers; exposure chambers are then covered and incubated in complete darkness for five days. Inhibition of root elongation is calculated as an EC50 (exposure concentration which yields a 50% reduction in root length relative to controls) upon termination of the test.
Keywords	Toxicity Germination Plants(Botany) Hazardous materials Waste disposal Mutagenicity tests Soil pollutants Superfund program Environmental monitoring
Source Agency	Environmental Protection Agency Office of Research and Development
NTIS Subject Category	68C - Solid Wastes Pollution & Control 57Y - Toxicology 57F - Cytology, Genetics, & Molecular Biology
Corporate Authors	Corvallis Environmental Research Lab., OR.; NSI Technology Services Corp., Corvallis, OR.
Supplemental Notes	Presented at Symposium on Use of Plants in Toxicity Assessment (1st), Atlanta, GA., April 16-21, 1989. Prepared in cooperation with NSI Technology Services Corp., Corvallis, OR.
Document Type	Technical Report
Title Note	Symposium paper.
NTIS Issue Number	199002