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Reproductive Toxicity Testing by Continuous Breeding Test Protocol in Swiss (CD-1) Mice,

PB89152425

Publication Date	1989
Personal Author	Heindel, J. J.; Lamb, J. C.; Chapin, R. E.; Gulati, D. K.; Hope, E.
Page Count	47
Abstract	'Reproductive Assessment by Continuous Breeding (RACB)' is a new standardized protocol designed for routine reproductive toxicity testing. Until recently, RACB was generally referred to as 'Fertility Assessment by Continuous Breeding (FACB),' is designed to improve the reliability and sensitivity of reproductive testing. Many RACB endpoints (fertility, pups per litter, pup weight, sex, and survival) are the same as in conventional EPA and FDA protocols. In addition, the RACB protocol tests fertility and reproductive outcome under constant challenge. The first generation animals (F0) are housed as breeding pairs continuously for 14 weeks. This approach not only increases the number of litters from each breeding pair, but also adds the dimension of time (progression of an adverse affect) to the evaluation of the reproductive performance. Thus, an early effect can be distinguished from a later, or progressive, effect. Standard multigeneration studies fail to provide such information unless more than one mating per generation is performed. This paper describes the standard methodology for conducting the RACB study in mice.
Keywords	Toxicology Breeding Reproduction(Biology) Fertility Reliability Sensitivity Exposure Lethal dosage Laboratory animals Mice Experimental design Germ cells Toxic substances Protocols Screening Dose-response relationships Occupational safety and health Body weight Fetal death Maternal mortality Teratogenesis
Source Agency	National Institute of Environmental Health Sciences
NTIS Subject Category	57Y - Toxicology
Corporate Authors	National Toxicology Program, Research Triangle Park, NC.; Research Triangle Inst., Research Triangle Park, NC.; Environmental Health Research and Testing, Inc., Lexington, KY.; Analytical Sciences, Inc., Research Triangle Park, NC.
Supplemental Notes	See also PB87-204491. Prepared in cooperation with Environmental Health Research and Testing, Inc., Lexington, KY., Research Triangle Inst., Research Triangle Park, NC., and Analytical Sciences, Inc., Research Triangle Park, NC.
Document Type	Technical Report
NTIS Issue Number	198911

Reproductive Toxicity Testing by Continuous Breeding Test Protocol in Swiss (CD-1) Mice,

Reproductive Toxicity Testing by Continuous Breeding Test Protocol in Swiss (CD-1) Mice,
PB89152425

Publication Date	1989
Personal Author	Heindel, J. J.; Lamb, J. C.; Chapin, R. E.; Gulati, D. K.; Hope, E.
Page Count	47
Abstract	'Reproductive Assessment by Continuous Breeding (RACB)' is a new standardized protocol designed for routine reproductive toxicity testing. Until recently, RACB was generally referred to as 'Fertility Assessment by Continuous Breeding (FACB),' is designed to improve the reliability and sensitivity of reproductive testing. Many RACB endpoints (fertility, pups per litter, pup weight, sex, and survival) are the same as in conventional EPA and FDA protocols. In addition, the RACB protocol tests fertility and reproductive outcome under constant challenge. The first generation animals (F0) are housed as breeding pairs continuously for 14 weeks. This approach not only increases the number of litters from each breeding pair, but also adds the dimension of time (progression of an adverse affect) to the evaluation of the reproductive performance. Thus, an early effect can be distinguished from a later, or progressive, effect. Standard multigeneration studies fail to provide such information unless more than one mating per generation is performed. This paper describes the standard methodology for conducting the RACB study in mice.
Keywords	Toxicology Breeding Reproduction(Biology) Fertility Reliability Sensitivity Exposure Lethal dosage Laboratory animals Mice Experimental design Germ cells Toxic substances Protocols Screening Dose-response relationships Occupational safety and health Body weight Fetal death Maternal mortality Teratogenesis
Source Agency	National Institute of Environmental Health Sciences
NTIS Subject Category	57Y - Toxicology
Corporate Authors	National Toxicology Program, Research Triangle Park, NC.; Research Triangle Inst., Research Triangle Park, NC.; Environmental Health Research and Testing, Inc., Lexington, KY.; Analytical Sciences, Inc., Research Triangle Park, NC.
Supplemental Notes	See also PB87-204491. Prepared in cooperation with Environmental Health Research and Testing, Inc., Lexington, KY., Research Triangle Inst., Research Triangle Park, NC., and Analytical Sciences, Inc., Research Triangle Park, NC.
Document Type	Technical Report
NTIS Issue Number	198911