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Effects of 4-Tertiary Butyl Catechol on Glutathione-Metabolizing Enzymes In vivo and In vitro.

PB88248349

Publication Date	1988
Personal Author	Kawashima, T.; Yonemoto, K.; Gellin, G. A.; Epstein, W. L.; Fukuyama, K.
Page Count	29
Abstract	The effects of noncytotoxic doses of 4-tertiary-butyl-catechol (98293) (TBC) on glutathione-S-transferase (GST) activity in the skin of male, 2-month-old Uscd-mice and B16-murine-melanoma cells in culture were investigated. These effects were studied in relation to changes in the activities of glutathione-reductase (GR) and gamma-glutamyl-transpeptidase (GGT) which are involved in pheomelanogenesis. Electron microscopic findings in the in-vivo studies demonstrated an altered form of melanosomes, classified as pheomelanosomes, in the TBC treated animals. Ear tissues demonstrated increased activities for all enzymes tested following TBC application. These increases were 27, 19, and 35 percent for GR, GST, and GGT, respectively. In-vitro studies demonstrated a dose-dependent decrease in the eumelanin content. GR, GGT, and GST activity in TBC-treated cells increased. The authors suggest that these findings may aid in the construction of an experimental model to investigate the molecular action of other more commonly used chemical depigmenting agents.
Keywords	Pyrocatechols Antioxidants Glutathione Occupational diseases Laboratory animals Melanoma Metabolism Electron microscopy Pigmentation disorders Melanocytes Butyl catechols Melanins CAS 9-82-93 Cultured cells Benzenediol/dimethylethyl Glutathione transferases Glutathione reductase Gamma-glutamyltransferase
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57B - Biochemistry 57U - Public Health & Industrial Medicine
Corporate Authors	California Univ., San Francisco. Dept. of Dermatology.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	198824

Effects of 4-Tertiary Butyl Catechol on Glutathione-Metabolizing Enzymes In vivo and In vitro.

Effects of 4-Tertiary Butyl Catechol on Glutathione-Metabolizing Enzymes In vivo and In vitro.
PB88248349

Publication Date	1988
Personal Author	Kawashima, T.; Yonemoto, K.; Gellin, G. A.; Epstein, W. L.; Fukuyama, K.
Page Count	29
Abstract	The effects of noncytotoxic doses of 4-tertiary-butyl-catechol (98293) (TBC) on glutathione-S-transferase (GST) activity in the skin of male, 2-month-old Uscd-mice and B16-murine-melanoma cells in culture were investigated. These effects were studied in relation to changes in the activities of glutathione-reductase (GR) and gamma-glutamyl-transpeptidase (GGT) which are involved in pheomelanogenesis. Electron microscopic findings in the in-vivo studies demonstrated an altered form of melanosomes, classified as pheomelanosomes, in the TBC treated animals. Ear tissues demonstrated increased activities for all enzymes tested following TBC application. These increases were 27, 19, and 35 percent for GR, GST, and GGT, respectively. In-vitro studies demonstrated a dose-dependent decrease in the eumelanin content. GR, GGT, and GST activity in TBC-treated cells increased. The authors suggest that these findings may aid in the construction of an experimental model to investigate the molecular action of other more commonly used chemical depigmenting agents.
Keywords	Pyrocatechols Antioxidants Glutathione Occupational diseases Laboratory animals Melanoma Metabolism Electron microscopy Pigmentation disorders Melanocytes Butyl catechols Melanins CAS 9-82-93 Cultured cells Benzenediol/dimethylethyl Glutathione transferases Glutathione reductase Gamma-glutamyltransferase
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57B - Biochemistry 57U - Public Health & Industrial Medicine
Corporate Authors	California Univ., San Francisco. Dept. of Dermatology.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	198824