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The National Technical Information Service acquires, indexes, abstracts, and archives the largest collection of U.S. government-sponsored technical reports in existence. The NTRL offers online, free and open access to these authenticated government technical reports. Technical reports and documents in its repository may be available online for free either from the issuing federal agency, the U.S. Government Publishing Office’s Federal Digital System website, or through search engines.

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Pathomechanisms of Chemically Induced Depigmentation,

PB88247077

Publication Date	1983
Personal Author	Gellin, G. A.
Page Count	11
Abstract	Analyses were made of skin from albino-mice, pigmented-mice, and melanoma cells. System-A detects DOPA, cysteinyldopas and related compounds. The melanoma cells demonstrated six peaks, pigmented ear skin showed four and albino ear skin only one. Using system-B, which detects indole derivatives, two peaks were observed. Butylated-hydroxytoluene (BHT) was added to the food given to mice. Four weeks after feeding BHT the pigment was sparse and irregular. Electron microscopy studies were carried out on human skin from patients before and after treatment with PUVA. After 2 weeks of treatment many melanosomes demonstrated irregular deposition of pigment which was ultrastructurally interpreted as the start of pheomelanogenesis. Skin biopsy of a black woman with depigmentation who wore rubber gloves while working at a hotel showed no melanocytes. Tests revealed melanosomes with a pheomelanogenesis-like ultrastructure. The author concludes that the basic methods needed to investigate chemically induced depigmentation have been developed.
Keywords	Occupational diseases Pigments Chromatographic analysis Melanoma Exposure Antioxidants Laboratory animals Chemical analysis Ultraviolet radiation Enzymes Skin pigmentation Pigmentation disorders Occupational safety and health Melanocytes Butylated hydroxytoluene Phenol/bis(dimethyl)-methyl) Benzenediol/dimethylethyl PUVA therapy
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57U - Public Health & Industrial Medicine 57O - Pathology 57E - Clinical Medicine 99A - Analytical Chemistry
Corporate Authors	National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	198823

Pathomechanisms of Chemically Induced Depigmentation,

Pathomechanisms of Chemically Induced Depigmentation,
PB88247077

Publication Date	1983
Personal Author	Gellin, G. A.
Page Count	11
Abstract	Analyses were made of skin from albino-mice, pigmented-mice, and melanoma cells. System-A detects DOPA, cysteinyldopas and related compounds. The melanoma cells demonstrated six peaks, pigmented ear skin showed four and albino ear skin only one. Using system-B, which detects indole derivatives, two peaks were observed. Butylated-hydroxytoluene (BHT) was added to the food given to mice. Four weeks after feeding BHT the pigment was sparse and irregular. Electron microscopy studies were carried out on human skin from patients before and after treatment with PUVA. After 2 weeks of treatment many melanosomes demonstrated irregular deposition of pigment which was ultrastructurally interpreted as the start of pheomelanogenesis. Skin biopsy of a black woman with depigmentation who wore rubber gloves while working at a hotel showed no melanocytes. Tests revealed melanosomes with a pheomelanogenesis-like ultrastructure. The author concludes that the basic methods needed to investigate chemically induced depigmentation have been developed.
Keywords	Occupational diseases Pigments Chromatographic analysis Melanoma Exposure Antioxidants Laboratory animals Chemical analysis Ultraviolet radiation Enzymes Skin pigmentation Pigmentation disorders Occupational safety and health Melanocytes Butylated hydroxytoluene Phenol/bis(dimethyl)-methyl) Benzenediol/dimethylethyl PUVA therapy
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57U - Public Health & Industrial Medicine 57O - Pathology 57E - Clinical Medicine 99A - Analytical Chemistry
Corporate Authors	National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	198823