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Tier II Mutagenic Screening of 13 NIOSH Priority Compounds: Individual Compound Report, N,N-Dimethylformamde.

PB83133900

Publication Date	1981
Personal Author	McGregor, D. B.
Page Count	198
Abstract	N,N-Dimethylformamide (68122) (NND) was subjected to a tier II mutagenic test screening program. The assays included unscheduled DNA synthesis (UDS) assay in human diploid fibroblasts with exposure of 3 hours duration and concentrations up to 9,220 micrograms per milliliter of culture medium; dominant lethal test in male rats with exposure to atmospheres containing 10 parts per million (ppm) or 400ppm NND; sperm abnormality test in male mice using the same exposure conditions; cytogenetic test in male and female rat bone marrow cells using the same exposure conditions or a single exposure of 7 hours duration; and sex linked recessive lethal test in Drosphila-melanogaster with exposure to an atmosphere of 400ppm for 2.25 hours. There was no increase in UDS in cells treated with NND nor were there effects attributable to NND in the dominant lethal test on pregnancy frequency, numbers of corpora lutea or implantations, or the frequency of early deaths. Abnormal sperm frequency was not affected, the frequencies of chromosomal aberrations were not increased in rat bone marrow cells, and sex linked recessive lethal frequency was not increased in Drosphila. The author concludes that NND was devoid of genetic effects.
Keywords	Toxicology Bioassay Screening Genetic diseases Deoxyribonucleic acids Exposure Concentration(Composition) Rats Laboratory animals Nitrogen organic compounds Formamide/N-N-dimethyl Mutagenesis CAS 68-12-2 Fibroblasts
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57Y - Toxicology 57F - Cytology, Genetics, & Molecular Biology
Corporate Authors	Inveresk Research International Ltd., Musselburgh (Scotland).; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	198305
Contract Number	PHS-NIOSH-210-78-0026

Tier II Mutagenic Screening of 13 NIOSH Priority Compounds: Individual Compound Report, N,N-Dimethylformamde.

Tier II Mutagenic Screening of 13 NIOSH Priority Compounds: Individual Compound Report, N,N-Dimethylformamde.
PB83133900

Publication Date	1981
Personal Author	McGregor, D. B.
Page Count	198
Abstract	N,N-Dimethylformamide (68122) (NND) was subjected to a tier II mutagenic test screening program. The assays included unscheduled DNA synthesis (UDS) assay in human diploid fibroblasts with exposure of 3 hours duration and concentrations up to 9,220 micrograms per milliliter of culture medium; dominant lethal test in male rats with exposure to atmospheres containing 10 parts per million (ppm) or 400ppm NND; sperm abnormality test in male mice using the same exposure conditions; cytogenetic test in male and female rat bone marrow cells using the same exposure conditions or a single exposure of 7 hours duration; and sex linked recessive lethal test in Drosphila-melanogaster with exposure to an atmosphere of 400ppm for 2.25 hours. There was no increase in UDS in cells treated with NND nor were there effects attributable to NND in the dominant lethal test on pregnancy frequency, numbers of corpora lutea or implantations, or the frequency of early deaths. Abnormal sperm frequency was not affected, the frequencies of chromosomal aberrations were not increased in rat bone marrow cells, and sex linked recessive lethal frequency was not increased in Drosphila. The author concludes that NND was devoid of genetic effects.
Keywords	Toxicology Bioassay Screening Genetic diseases Deoxyribonucleic acids Exposure Concentration(Composition) Rats Laboratory animals Nitrogen organic compounds Formamide/N-N-dimethyl Mutagenesis CAS 68-12-2 Fibroblasts
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57Y - Toxicology 57F - Cytology, Genetics, & Molecular Biology
Corporate Authors	Inveresk Research International Ltd., Musselburgh (Scotland).; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
NTIS Issue Number	198305
Contract Number	PHS-NIOSH-210-78-0026