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Cytogenetic Evaluation of Bone Marrow Cells in the Rat Following Long Term Inhalation Exposure to Nitrous Oxide Plus Halothane.

PB82164849

Publication Date	1970
Page Count	27
Abstract	The mutagenic potential of long-term exposure of 120 male Sprague-Dawley rats to nitrous oxide is measured by observing aberrations in bone marrow cell chromosomes. Forty rats serve as controls, 40 are exposed to 50 ppm nitrous exide plus 1 ppm halothane, and 40 are exposed to 500 ppm nitrous oxide plus 10 ppm halothane, for 7 hours a day, 5 days a week or 52 weeks. The most frequently found aberration is the chromatid gap. There is a significant increase in the number of gaps and this increase is dose-related. Chromatid breaks are the next most frequent aberrations observed. Chromosomal breaks are comparable among all groups. Chromosomal markers are significantly increased in the group receiving 500 ppm nitrous oxide. The incidence of polyploidy is comparable among control and treated groups. Fifty-nine percent of the animals in the first treatment group show aberrant cells; 68% in the second treatment group. It is concluded that exposure to 500 ppm nitrous oxide plus 10 ppm halothane can cause chromosomal damage to bone marrow cells in the rat.
Keywords	Toxicology Nitrogen oxide(N2O) Rats Males Laboratory animals Exposure Mutagens Chromosomes Bone marrow Dosage Bioassay Cells(Biology) Respiration Air pollution Mutagenesis Air pollution effects(Animals) Toxic substances Halothanes
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57Y - Toxicology 57O - Pathology 68A - Air Pollution & Control
Corporate Authors	Hazleton Labs. America, Inc., Vienna, VA.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
Title Note	Final rept.
NTIS Issue Number	198212
Contract Number	PHS-CDC-99-74-46

Cytogenetic Evaluation of Bone Marrow Cells in the Rat Following Long Term Inhalation Exposure to Nitrous Oxide Plus Halothane.

Cytogenetic Evaluation of Bone Marrow Cells in the Rat Following Long Term Inhalation Exposure to Nitrous Oxide Plus Halothane.
PB82164849

Publication Date	1970
Page Count	27
Abstract	The mutagenic potential of long-term exposure of 120 male Sprague-Dawley rats to nitrous oxide is measured by observing aberrations in bone marrow cell chromosomes. Forty rats serve as controls, 40 are exposed to 50 ppm nitrous exide plus 1 ppm halothane, and 40 are exposed to 500 ppm nitrous oxide plus 10 ppm halothane, for 7 hours a day, 5 days a week or 52 weeks. The most frequently found aberration is the chromatid gap. There is a significant increase in the number of gaps and this increase is dose-related. Chromatid breaks are the next most frequent aberrations observed. Chromosomal breaks are comparable among all groups. Chromosomal markers are significantly increased in the group receiving 500 ppm nitrous oxide. The incidence of polyploidy is comparable among control and treated groups. Fifty-nine percent of the animals in the first treatment group show aberrant cells; 68% in the second treatment group. It is concluded that exposure to 500 ppm nitrous oxide plus 10 ppm halothane can cause chromosomal damage to bone marrow cells in the rat.
Keywords	Toxicology Nitrogen oxide(N2O) Rats Males Laboratory animals Exposure Mutagens Chromosomes Bone marrow Dosage Bioassay Cells(Biology) Respiration Air pollution Mutagenesis Air pollution effects(Animals) Toxic substances Halothanes
Source Agency	National Institute for Occupational Safety and Health
NTIS Subject Category	57Y - Toxicology 57O - Pathology 68A - Air Pollution & Control
Corporate Authors	Hazleton Labs. America, Inc., Vienna, VA.; National Inst. for Occupational Safety and Health, Cincinnati, OH.
Document Type	Technical Report
Title Note	Final rept.
NTIS Issue Number	198212
Contract Number	PHS-CDC-99-74-46