Abstract |
The need, material selection, characterization, certification, and uses of the fluorescence Standard Reference Material, quinine sulfate dihydrate, have been discussed. The emission spectrum for quinine sulfate dihydrate in 0.1 mol/L HClO4 has been measured on the NBS reference spectroradiometer. The technical emission spectrum has been corrected for the instrumental parameters of the spectral responsitivity of the detection system, photomultiplier tube nonlinearity, bandpass, monochromator wavelength error, and further corrections for the sample parameters of solvent refractive index and cell window transmittance were applied to obtain the molecular emission spectra. The purity and the stability of the SRM quinine sulfate dihydrate and the effect of solute concentration, solvent acid, acid concentration, excitation wavelength, oxygen quenching, temperature and polarized exciting radiation on the emission spectrum, and in part, on the absorbance spectrum, photon yield, and fluorescence lifetime also have been discussed. |