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Organic Dust Epithelial PKC Activation and Airway Disease.

PB2013107690

Publication Date	2010
Personal Author	Romberger, D. J.; Toews, M.; West, W.; Whatt, T.; Meza, J.; Parinandi, N.
Page Count	13
Abstract	Exposure to organic dusts is a cause of airway disease, including chronic obstructive pulmonary disease (COPD). As many as 20% of COPD cases are attributed to occupational exposures. In rural areas, an important source of dust exposure occurs in hog concentrated animal feeding operations (CAFOs). Persons exposed to hog CAFOs have airway inflammation and an increased incidence of COPD. Although many substances are present in hog barn dust that induce inflammation, actual mechanisms leading to COPD are not well defined. Understanding mechanisms of hog barn dust-induced airway disease is relevant in developing both targeted treatment and prevention strategies. Epithelial cells within the airways respond to inhaled agents with the release of cytokines that recruit and activate inflammatory cells and expression of molecules that serve as receptors and ligands for interactions with other cells. Previously, we observed that hog barn dust extract (HDE) augments human airway epithelial protein kinase C (PKC) activation, resulting in IL-8 and IL-6 release and increased ICAM-1 expression, mediating inflammatory cell adhesion to airway epithelium in vitro. The objective of this proposal was to define mechanisms by which hog barn dust activates epithelial cell PKC and the role of PKC in airway inflammation associated with chronic bronchitis occurring in confinement facility workers and to determine the role of hog barn dust-related lysophosphatidic acid (LPA), an important lipid mediator, in modulating dust effects on PKC and inflammatory responses.
Keywords	Dusts Air pollution control Environmental exposure pathway Lung diseases Agricultural industry Animals Farmers Inhalation Molecular biology Pulmonary system disorders Respiratory system disorders Rural areas Work environment
Source Agency	National Institute for Occupational Safety and Health
Corporate Authors	Nebraska Univ. Medical Center, Omaha. Dept. of Internal Medicine.; National Inst. for Occupational Safety and Health, Washington, DC.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	201317

Organic Dust Epithelial PKC Activation and Airway Disease.

Organic Dust Epithelial PKC Activation and Airway Disease.
PB2013107690

Publication Date	2010
Personal Author	Romberger, D. J.; Toews, M.; West, W.; Whatt, T.; Meza, J.; Parinandi, N.
Page Count	13
Abstract	Exposure to organic dusts is a cause of airway disease, including chronic obstructive pulmonary disease (COPD). As many as 20% of COPD cases are attributed to occupational exposures. In rural areas, an important source of dust exposure occurs in hog concentrated animal feeding operations (CAFOs). Persons exposed to hog CAFOs have airway inflammation and an increased incidence of COPD. Although many substances are present in hog barn dust that induce inflammation, actual mechanisms leading to COPD are not well defined. Understanding mechanisms of hog barn dust-induced airway disease is relevant in developing both targeted treatment and prevention strategies. Epithelial cells within the airways respond to inhaled agents with the release of cytokines that recruit and activate inflammatory cells and expression of molecules that serve as receptors and ligands for interactions with other cells. Previously, we observed that hog barn dust extract (HDE) augments human airway epithelial protein kinase C (PKC) activation, resulting in IL-8 and IL-6 release and increased ICAM-1 expression, mediating inflammatory cell adhesion to airway epithelium in vitro. The objective of this proposal was to define mechanisms by which hog barn dust activates epithelial cell PKC and the role of PKC in airway inflammation associated with chronic bronchitis occurring in confinement facility workers and to determine the role of hog barn dust-related lysophosphatidic acid (LPA), an important lipid mediator, in modulating dust effects on PKC and inflammatory responses.
Keywords	Dusts Air pollution control Environmental exposure pathway Lung diseases Agricultural industry Animals Farmers Inhalation Molecular biology Pulmonary system disorders Respiratory system disorders Rural areas Work environment
Source Agency	National Institute for Occupational Safety and Health
Corporate Authors	Nebraska Univ. Medical Center, Omaha. Dept. of Internal Medicine.; National Inst. for Occupational Safety and Health, Washington, DC.
Supplemental Notes	Sponsored by National Inst. for Occupational Safety and Health, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	201317