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Assessment of Health Impacts of Particulate Matter from Indoor Air Sources Phase I: Development of In Vitro Methodology. Final Report.

PB2010114915

Publication Date	2010
Personal Author	Matsumura, F.; Vogel, C. F.; Kobayashi, R.; Liu, X.; Wong, P.
Page Count	137
Abstract	The overall objective of this study was to develop approaches to assess the toxicities of several major indoor particulate matter (PM) source samples by using human in vitro cell models with a focus on inflammatory and oxidative stress responses. In the pilot study, the capacity of extracts from incense PM to stimulate inflammatory marker production in four in vitro human cell models was evaluated. The U937 macrophage cell line was the most sensitive of the test models followed by the NCI-H441 bronchiolar Clara cell line. PM toxicity from cooking activities, candle burning, wood burning, and incense burning was assessed in both cell models. All indoor PM sample source types had some positive response in either or both of the human cell lines with incense producing the largest responses. In further analytical studies, it was determined that incense PM contained high levels of PAHs while wood smoke had lower levels. Incense also was found to contain many other compounds such as vanillin which may contribute to its high toxicity. Wood smoke yielded hydrocarbons containing a series of siloxanes. Cooking PM sample from the stir frying contained mainly hydrocarbons related to the oil used. The candle samples consisted of numerous hydrocarbons such as alkanes and alkenes.
Keywords	Particulates Indoor air pollution Toxicity In vitro Health assessments Figures Biological characterization Chemical characterization Polycyclic aromatic hydrocarbons (PAHs) Tables (Data) Recommendations Indoor particulate matter (PM) sources Human cell models
Source Agency	California Air Resources Board Resources Agency
Corporate Authors	California Univ., Davis. Dept. of Environmental Toxicology.; California Air Resources Board, Sacramento, CA.
Supplemental Notes	Sponsored by California Air Resources Board, Sacramento, CA.
Document Type	Technical Report
NTIS Issue Number	201024

Assessment of Health Impacts of Particulate Matter from Indoor Air Sources Phase I: Development of In Vitro Methodology. Final Report.

Assessment of Health Impacts of Particulate Matter from Indoor Air Sources Phase I: Development of In Vitro Methodology. Final Report.
PB2010114915

Publication Date	2010
Personal Author	Matsumura, F.; Vogel, C. F.; Kobayashi, R.; Liu, X.; Wong, P.
Page Count	137
Abstract	The overall objective of this study was to develop approaches to assess the toxicities of several major indoor particulate matter (PM) source samples by using human in vitro cell models with a focus on inflammatory and oxidative stress responses. In the pilot study, the capacity of extracts from incense PM to stimulate inflammatory marker production in four in vitro human cell models was evaluated. The U937 macrophage cell line was the most sensitive of the test models followed by the NCI-H441 bronchiolar Clara cell line. PM toxicity from cooking activities, candle burning, wood burning, and incense burning was assessed in both cell models. All indoor PM sample source types had some positive response in either or both of the human cell lines with incense producing the largest responses. In further analytical studies, it was determined that incense PM contained high levels of PAHs while wood smoke had lower levels. Incense also was found to contain many other compounds such as vanillin which may contribute to its high toxicity. Wood smoke yielded hydrocarbons containing a series of siloxanes. Cooking PM sample from the stir frying contained mainly hydrocarbons related to the oil used. The candle samples consisted of numerous hydrocarbons such as alkanes and alkenes.
Keywords	Particulates Indoor air pollution Toxicity In vitro Health assessments Figures Biological characterization Chemical characterization Polycyclic aromatic hydrocarbons (PAHs) Tables (Data) Recommendations Indoor particulate matter (PM) sources Human cell models
Source Agency	California Air Resources Board Resources Agency
Corporate Authors	California Univ., Davis. Dept. of Environmental Toxicology.; California Air Resources Board, Sacramento, CA.
Supplemental Notes	Sponsored by California Air Resources Board, Sacramento, CA.
Document Type	Technical Report
NTIS Issue Number	201024