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Four Final Mutagenicity Reports Regarding Phthalate Esters.

OTS0510527

Publication Date	2000
Page Count	108
Abstract	The ability of butyl benzyl phthalate to induce specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells (Mouse Lymphoma Mutagenicity Assay) was evaluated in the presence and absence of Aroclor-induced rat liver S-9 metabolic activation. Based on preliminary toxicity tests, 16 nonactivated cultures treated from 6.25nl/ml to 40.0nl/ml were cloned, producing a range of 83 - 2.5% relative growth. Twenty S-9 activated cultures treated from 200 to 1400nl/ml were cloned, producing a range of 65.7 - 1.0% relative growth. None of the cultures produced mutant frequencies significantly greater than the solvent control (acetone). Di-(n-hexyl, n-octyl, n-decyl) phthalate (DHODP), dimethyl phthalate and di-n-butyl phthalate were also evaluated in the Mouse Lymphoma Mutagenicity Assay in the same manner as above. Based on preliminary toxicity tests, 10 nonactivated cultures treated from 2500 nl DHODP/ml to 8000 nl/ml were cloned, producing a relative growth range of 43.1 - 2.4%. Ten S-9 activated cultures treated from 150 nl DHODP/ml to 400 nl/ml were cloned, producing a relative growth range of 82.2 - 9.2&. With the exception of the 150 nl/ml activated culture, all cultures with or withour metabolic activation produced mutant frequencies greater than the solvent control. In the next study, based on preliminary toxicity tests, 10 nonactivated cultures treated with 15 nl dimethyl phthalate/ml to 40 nl/ml were cloned, producing a relative growth range of 83 - 11.3%. Ten S-9 activated cultures were then treated with 600 nl dimethyl phthalate/ml to 1400 nl/ml, producing a relative growth range of 65.7 - 6.2%. None of the dimethyl phthalate cultures produced mutant frequencies significantly greater than the solvent control. Also based on preliminary toxicity tests, 4 nonactivated cultures treated with di-n-butyl phthalate at concentrations of 25 nl/ml and 50 nl/ml produced relative growth ranges of 79.2 - 48.7%. Six S-9 activated cultures were then treated with 25 - 75 nl/ml, producing relative growth ranges of 91.1 - 6.9%. All of the S-9 activated di-n-butyl phthalate cultures produced mutant frequencies greater than the solvent controls.
Keywords	Toxicology Health effects Phthalate Esters Genotoxicity Gene Mutations Mammals Mice In Vitro Toxic substances Laboratory animals CAS No. 84-74-2 CAS No. 85-68-7 CAS No. 131-11-3 CAS No. 68515-51-5
Source Agency	Office of Toxic Substances
Corporate Authors	Hazleton Biotechnologies, Kensington, MD.; Chemical Manufacturers Association, Washington, DC.; Environmental Protection Agency, Washington, DC. Office of Toxic
Supplemental Notes	The quality of the documents listed in the Office of Toxic Substances database may not meet usual NTIS standards but are included to further the opportunity for the scientific and technical community to locate materials which may not be available elsewhere. The content is the responsibility of OTS. Prepared in cooperation with Chemical Manufacturers Association, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200822

Four Final Mutagenicity Reports Regarding Phthalate Esters.

Four Final Mutagenicity Reports Regarding Phthalate Esters.
OTS0510527

Publication Date	2000
Page Count	108
Abstract	The ability of butyl benzyl phthalate to induce specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells (Mouse Lymphoma Mutagenicity Assay) was evaluated in the presence and absence of Aroclor-induced rat liver S-9 metabolic activation. Based on preliminary toxicity tests, 16 nonactivated cultures treated from 6.25nl/ml to 40.0nl/ml were cloned, producing a range of 83 - 2.5% relative growth. Twenty S-9 activated cultures treated from 200 to 1400nl/ml were cloned, producing a range of 65.7 - 1.0% relative growth. None of the cultures produced mutant frequencies significantly greater than the solvent control (acetone). Di-(n-hexyl, n-octyl, n-decyl) phthalate (DHODP), dimethyl phthalate and di-n-butyl phthalate were also evaluated in the Mouse Lymphoma Mutagenicity Assay in the same manner as above. Based on preliminary toxicity tests, 10 nonactivated cultures treated from 2500 nl DHODP/ml to 8000 nl/ml were cloned, producing a relative growth range of 43.1 - 2.4%. Ten S-9 activated cultures treated from 150 nl DHODP/ml to 400 nl/ml were cloned, producing a relative growth range of 82.2 - 9.2&. With the exception of the 150 nl/ml activated culture, all cultures with or withour metabolic activation produced mutant frequencies greater than the solvent control. In the next study, based on preliminary toxicity tests, 10 nonactivated cultures treated with 15 nl dimethyl phthalate/ml to 40 nl/ml were cloned, producing a relative growth range of 83 - 11.3%. Ten S-9 activated cultures were then treated with 600 nl dimethyl phthalate/ml to 1400 nl/ml, producing a relative growth range of 65.7 - 6.2%. None of the dimethyl phthalate cultures produced mutant frequencies significantly greater than the solvent control. Also based on preliminary toxicity tests, 4 nonactivated cultures treated with di-n-butyl phthalate at concentrations of 25 nl/ml and 50 nl/ml produced relative growth ranges of 79.2 - 48.7%. Six S-9 activated cultures were then treated with 25 - 75 nl/ml, producing relative growth ranges of 91.1 - 6.9%. All of the S-9 activated di-n-butyl phthalate cultures produced mutant frequencies greater than the solvent controls.
Keywords	Toxicology Health effects Phthalate Esters Genotoxicity Gene Mutations Mammals Mice In Vitro Toxic substances Laboratory animals CAS No. 84-74-2 CAS No. 85-68-7 CAS No. 131-11-3 CAS No. 68515-51-5
Source Agency	Office of Toxic Substances
Corporate Authors	Hazleton Biotechnologies, Kensington, MD.; Chemical Manufacturers Association, Washington, DC.; Environmental Protection Agency, Washington, DC. Office of Toxic
Supplemental Notes	The quality of the documents listed in the Office of Toxic Substances database may not meet usual NTIS standards but are included to further the opportunity for the scientific and technical community to locate materials which may not be available elsewhere. The content is the responsibility of OTS. Prepared in cooperation with Chemical Manufacturers Association, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200822