| Abstract |
The ability of 1K [di(heptyl, nonyl, undecyl) phthalate] to induce morphological transformation in vitro was evaluated in the Balb/C-3T3 mouse cell line (Cell Transformation Assay). Based on preliminary determinations of cytotoxicity, the test compound was evaluated at concentrations of 0, 60, 190, 600, 1900, and 6000 nl/ml culture medium. Cell survival was concentration-related, ranging from 88 to 12.4% over the range of concentrations tested. The treatment did not increase the frequency of cell transformation, suggesting that 1K was not carcinogenic in the mouse under the conditions of this assay. The ability of diisodecyl phthalate to induce morphological transformation in the BALB/c-3T3 mouse cell line (Cell Transformation Assay) was evaluated. The test material was relatively nontoxic at treatment doses below and above the solubility limit in culture medium (1000 to 5000 nl/ml). Diisodecyl phthalate at concentrations of 200 to 20,000 nl/ml did not induce statistically significant increases in transforming activity, and 51 to 74% survival was observed in simultaneous colony survival assays. Diundecyl phthalate was tested in the cell transformation assay in vitro on the Balb/c-3T3 mouse cell line. Preliminary toxicity assays in plastic culture vessels did not produce a clear dose response relationship; thus five arbitrary doses at and above the test article's solubility (ranging from 4000 to 100000 nl/ml) were chosen for the transformation assay, resulting in cell survival ranging from 64 to 86% relative to the control (culture medium). Assays conducted in glass bottle culture vessels did not a produce a clear dose response; thus 5 arbitrary doses were chosen both above and below the solubility limit (ranging from 400 to 40000 nl/ml), resulting in cell survival ranging from 44 to 86% relative to the control (culture medium). None of the treatments in plastic vessels produced significantly greater transformation frequencies relative to the negative control. At a concentration of 12,650 nl/ml in the glass culture flasks, a statistically significant (.02<p<.05) increase in the number of transformed foci was observed. Dimethyl phthalate was evaluated for cytotoxicity in the mouse lymphoma L5178Y cell line, in the presence and absence of rat liver S9 metabolic activation. All cultures were treated in duplicate with concentrations of 9.77, 19.50, 39.10, 78.10, 156.00, 313.00, 625.00, 1250.00, 2500.00 or 5000.00 nl/ml, and growth was determined at 24 and 48 hours after initiation of the treatment. Under activated and non-activated conditions, dimethyl phthalate was soluble up to 5000 nl/ml. Under non-activated conditions, treatments at 625 nl/ml were weakly toxic (69.9% of solvent (acetone) control suspension growth) and treatments at 1250 nl/mlor higher were 100% lethal. Under activated conditions, treatments at 625 nl/ml were weakly toxic (75.5% average relative suspension growth), 1 treatment at 1250 nl/ml was 100% lethal and a duplicate treatment at the same concentration was highly toxic (22.9% average relative suspension growth), and treatment at concentrations higher than 1250 nl/ml were 100% lethal. Di-n-butyl phthalate was evaluated for cytotoxicity in the mouse lymphoma L5178Y cell line, in the presence and absence of rat liver S9 metabolic activation. All cultures were treated in duplicate with concentrations of 9.77, 19.50, 39.10, 78.10, 156.00, 313.00, 625.00, 1250.00, 2500.00, or 5000.00 nl/ml, and growth was determined at 24 and 48 hours after initiation of the treatment. Under non-activated conditions, di-n-butyl |
