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Microbial Aldonolactone Formation and Hydrolysis: Kinetic and Bioenergetic Aspects.

N9229735

Publication Date	1991
Personal Author	Noorman, H. J.; Rakels, J. L. L.; Kuenen, J. G.; Luyben, K. C. A. M.; Heijnen, J. J.
Page Count	30
Abstract	During growth in mixtures of acetate and aldose sugars, the growth yield of the bacterium Acinetobacter caloaceticus was increased when aldonolactone hydrolysis occurred. The underlying mechanism was investigated by studying the relationship between cellular yield and lactone hydrolysis in a quantitative way. The literature on the kinetics of acid formation from aldonolactones was reviewed. The reaction pattern is composed of lactone isomerization and hydrolysis steps which occur in series. In the acid pH range, lactone interconversion is the slowest step, whereas above pH 7 hydrolysis is rate/limiting for acid formation. Only for D-gluconolactone could a detailed mechanistic model be constructed. For a number of other aldonolactones including D-xylono and D-galactono lactone, an empirical kinetic equation was derived. The rate constants of these lactones are much smaller than for gluconolactone. The kinetic model of the hydrolysis of xylonolactone was tested, using the electrical charge balance in the Acinetobacter system. The published biomass yield data and the rate of lactone hydrolysis were compared. The observed relation seems to be only apparent. The electrical charge balance in the study of processes in which charged compounds are being converted is shown to be powerful.
Keywords	Bacteriology Hydrolysis Kinetic equations Sugars Analysis (Mathematics) Culture techniques Acetates Biomass Cells (Biology) Kinetics Lactose Foreign technology Aldonolactone
Source Agency	NASA Foreign Exchange Program
NTIS Subject Category	57B - Biochemistry 57K - Microbiology
Corporate Authors	Technische Univ. Delft (Netherlands).
Supplemental Notes	In Its Methodology on Monitoring and Modelling of Microbial Metabolism p 97-126.
Document Type	Analytic Daughter
NTIS Issue Number	199223

Microbial Aldonolactone Formation and Hydrolysis: Kinetic and Bioenergetic Aspects.

Microbial Aldonolactone Formation and Hydrolysis: Kinetic and Bioenergetic Aspects.
N9229735

Publication Date	1991
Personal Author	Noorman, H. J.; Rakels, J. L. L.; Kuenen, J. G.; Luyben, K. C. A. M.; Heijnen, J. J.
Page Count	30
Abstract	During growth in mixtures of acetate and aldose sugars, the growth yield of the bacterium Acinetobacter caloaceticus was increased when aldonolactone hydrolysis occurred. The underlying mechanism was investigated by studying the relationship between cellular yield and lactone hydrolysis in a quantitative way. The literature on the kinetics of acid formation from aldonolactones was reviewed. The reaction pattern is composed of lactone isomerization and hydrolysis steps which occur in series. In the acid pH range, lactone interconversion is the slowest step, whereas above pH 7 hydrolysis is rate/limiting for acid formation. Only for D-gluconolactone could a detailed mechanistic model be constructed. For a number of other aldonolactones including D-xylono and D-galactono lactone, an empirical kinetic equation was derived. The rate constants of these lactones are much smaller than for gluconolactone. The kinetic model of the hydrolysis of xylonolactone was tested, using the electrical charge balance in the Acinetobacter system. The published biomass yield data and the rate of lactone hydrolysis were compared. The observed relation seems to be only apparent. The electrical charge balance in the study of processes in which charged compounds are being converted is shown to be powerful.
Keywords	Bacteriology Hydrolysis Kinetic equations Sugars Analysis (Mathematics) Culture techniques Acetates Biomass Cells (Biology) Kinetics Lactose Foreign technology Aldonolactone
Source Agency	NASA Foreign Exchange Program
NTIS Subject Category	57B - Biochemistry 57K - Microbiology
Corporate Authors	Technische Univ. Delft (Netherlands).
Supplemental Notes	In Its Methodology on Monitoring and Modelling of Microbial Metabolism p 97-126.
Document Type	Analytic Daughter
NTIS Issue Number	199223