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Inhalation Reproductive Toxicology Studies: Sperm Morphology Study of n-Hexane in B6C3F1 Mice: Final Report.

DE89000262

Publication Date	1988
Personal Author	Mast, T. J.; Hackett, P. L.; Decker, J. R.; Westerberg, R. B.; Sasser, L. B.
Page Count	146
Abstract	The straight-chain hydrocarbon, n-hexane, is a volatile, ubiquitous solvent routinely used in industrial environments. Although myelinated nerve tissue is the primary target organ of hexane, the testes have also been identified as being sensitive to hexacarbon exposure. The objective of this study was to evaluate the epididymal sperm morphology of male B6D3F1 mice 5 weeks after exposure to 0, 200, 1000, or 5000 ppM n-hexane, 20 h/day for 5 consecutive days. Two concurrent positive control groups of animals were injected intraperitoneally with either 200 or 250 mg/kg ethyl methanesulfonate, a known mutagen, once each day for 5 consecutive days. The mice were weighed just prior to the first day of exposure and at weekly intervals until sacrifice. During the fifth post-exposure week the animals were killed and examined for gross lesions of the reproductive tract and suspensions of the epididymal sperm were prepared for morphological evaluations. The appearance and behavior of the mice were unremarkable throughout the experiment and there were no deaths. No evidence of lesions in any organ was noted at sacrifice. Mean body weights of male mice exposed to n-hexane were not significantly different from those for the 0-ppM animals at any time during the study. Analyses of the sperm morphology data obtained 5 weeks post-exposure (the only time point examined) indicated that exposure of male mice to relatively high concentrations of n-hexane vapor for 5 days produced no significant effects on the morphology of sperm relative to that of the 0-ppM control group. 24 refs., 2 figs., 7 tabs. (ERA citation 13:057614)
Keywords	Hexane Inhalation Air Pollution Biological Effects Dose-Response Relationships Evaluation Exhalation Kinetics Lethal Doses Metabolism Mice Pharmacology Progress Report Reproduction Sex Dependence Air pollution effects(Animals) ERDA/560300
Source Agency	Technical Information Center Oak Ridge Tennessee
NTIS Subject Category	57Y - Toxicology 57Z - Zoology 68A - Air Pollution & Control 68G - Environmental Health & Safety
Corporate Authors	Battelle Pacific Northwest Labs., Richland, WA.; Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	198907
Contract Number	AC06-76RL01830

Inhalation Reproductive Toxicology Studies: Sperm Morphology Study of n-Hexane in B6C3F1 Mice: Final Report.

Inhalation Reproductive Toxicology Studies: Sperm Morphology Study of n-Hexane in B6C3F1 Mice: Final Report.
DE89000262

Publication Date	1988
Personal Author	Mast, T. J.; Hackett, P. L.; Decker, J. R.; Westerberg, R. B.; Sasser, L. B.
Page Count	146
Abstract	The straight-chain hydrocarbon, n-hexane, is a volatile, ubiquitous solvent routinely used in industrial environments. Although myelinated nerve tissue is the primary target organ of hexane, the testes have also been identified as being sensitive to hexacarbon exposure. The objective of this study was to evaluate the epididymal sperm morphology of male B6D3F1 mice 5 weeks after exposure to 0, 200, 1000, or 5000 ppM n-hexane, 20 h/day for 5 consecutive days. Two concurrent positive control groups of animals were injected intraperitoneally with either 200 or 250 mg/kg ethyl methanesulfonate, a known mutagen, once each day for 5 consecutive days. The mice were weighed just prior to the first day of exposure and at weekly intervals until sacrifice. During the fifth post-exposure week the animals were killed and examined for gross lesions of the reproductive tract and suspensions of the epididymal sperm were prepared for morphological evaluations. The appearance and behavior of the mice were unremarkable throughout the experiment and there were no deaths. No evidence of lesions in any organ was noted at sacrifice. Mean body weights of male mice exposed to n-hexane were not significantly different from those for the 0-ppM animals at any time during the study. Analyses of the sperm morphology data obtained 5 weeks post-exposure (the only time point examined) indicated that exposure of male mice to relatively high concentrations of n-hexane vapor for 5 days produced no significant effects on the morphology of sperm relative to that of the 0-ppM control group. 24 refs., 2 figs., 7 tabs. (ERA citation 13:057614)
Keywords	Hexane Inhalation Air Pollution Biological Effects Dose-Response Relationships Evaluation Exhalation Kinetics Lethal Doses Metabolism Mice Pharmacology Progress Report Reproduction Sex Dependence Air pollution effects(Animals) ERDA/560300
Source Agency	Technical Information Center Oak Ridge Tennessee
NTIS Subject Category	57Y - Toxicology 57Z - Zoology 68A - Air Pollution & Control 68G - Environmental Health & Safety
Corporate Authors	Battelle Pacific Northwest Labs., Richland, WA.; Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	198907
Contract Number	AC06-76RL01830