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BAC-FISH Assays Delineate Complex Chromosomal Rearrangements in a Case of Post-Chernobyl Childhood Thyroid Cancer.

DE2010983040

Publication Date	2009
Personal Author	Kwan, J.; Baumgartner, A.; Weier, H. U. G.; Lu, C. M.; Wang, M.
Page Count	27
Abstract	Structural chromosome aberrations are known hallmarks of many solid tumors. In the papillary form of thyroid cancer (PTC), for example, activation of the receptor tyrosine kinase (RTK) genes, RET and neurotrophic tyrosine kinase receptor type I (NTRK1) by intra- and interchromosomal rearrangements has been suggested as a cause of the disease. However, many phenotypically similar tumors do not carry an activated RET or NTRK-1 gene or express abnormal ret or NTRK-1 transcripts. Thus, we hypothesize that other cellular RTK-type genes are aberrantly expressed in these tumors. Using fluorescence in situ hybridization-based methods, we are studying karyotype changes in a relatively rare subgroup of PTCs, i.e., tumors that arose in children following the 1986 nuclear accident in Chernobyl, Ukraine. Here, we report our technical developments and progress in deciphering complex chromosome aberrations in case S48TK, an aggressively growing PTC cell line, which shows an unusual high number of unbalanced translocations.
Keywords	Thyroid cancer Children Radiation Chromosome aberration Bacterial artificial chromosome Chernobyl(Ukraine) Toxicity
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Lawrence Berkeley National Lab, CA. Life Sciences Div.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	201108
Contract Number	DE-AC02-05CH11231

BAC-FISH Assays Delineate Complex Chromosomal Rearrangements in a Case of Post-Chernobyl Childhood Thyroid Cancer.

BAC-FISH Assays Delineate Complex Chromosomal Rearrangements in a Case of Post-Chernobyl Childhood Thyroid Cancer.
DE2010983040

Publication Date	2009
Personal Author	Kwan, J.; Baumgartner, A.; Weier, H. U. G.; Lu, C. M.; Wang, M.
Page Count	27
Abstract	Structural chromosome aberrations are known hallmarks of many solid tumors. In the papillary form of thyroid cancer (PTC), for example, activation of the receptor tyrosine kinase (RTK) genes, RET and neurotrophic tyrosine kinase receptor type I (NTRK1) by intra- and interchromosomal rearrangements has been suggested as a cause of the disease. However, many phenotypically similar tumors do not carry an activated RET or NTRK-1 gene or express abnormal ret or NTRK-1 transcripts. Thus, we hypothesize that other cellular RTK-type genes are aberrantly expressed in these tumors. Using fluorescence in situ hybridization-based methods, we are studying karyotype changes in a relatively rare subgroup of PTCs, i.e., tumors that arose in children following the 1986 nuclear accident in Chernobyl, Ukraine. Here, we report our technical developments and progress in deciphering complex chromosome aberrations in case S48TK, an aggressively growing PTC cell line, which shows an unusual high number of unbalanced translocations.
Keywords	Thyroid cancer Children Radiation Chromosome aberration Bacterial artificial chromosome Chernobyl(Ukraine) Toxicity
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Lawrence Berkeley National Lab, CA. Life Sciences Div.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	201108
Contract Number	DE-AC02-05CH11231