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Exosporium of B. Cereus Contains a Binding Site for gC1qR/p33: Implication in Spore Attachment and/or Entry.

DE2008924425

Publication Date	2008
Personal Author	Ghebrehiwet, B.; Wong, S. S.; Warren, J. B.; Titmus, M. A.
Page Count	19
Abstract	B. cereus, is a member of a genus of aerobic, gram-positive, spore-forming rod-like bacilli, which includes the deadly, B. anthracis. Preliminary experiments have shown that gC1qR binds to B. cereus spores that have been attached to microtiter plates. The present studies were therefore undertaken, to examine if cell surface gC1qR plays a role in B. cereus spore attachment and/or entry. Monolayers of human colon carcinoma (Caco-2) and lung cells were grown to confluency on 6 mm coverslips in shell vials with gentle swirling in a shaker incubator. Then, 2 (micro)l of a suspension of strain SB460 B.cereus spores (3x10(sup 8)/ml, in sterile water), were added and incubated (1-4 h; 36(sup 0) C) in the presence or absence of anti-gC1qR mAb-carbon nanoloops. Examination of these cells by EM revealed that: (1) When B. cereus endospores contacted the apical Caco-2 cell surface, or lung cells, gClqR was simultaneously detectable, indicating upregulation of the molecule. (2) In areas showing spore contact with the cell surface, gClqR expression was often adjacent to the spores in association with microvilli (Caco-2 cells) or cytoskeletal projections (lung cells). (3) Furthermore, the exosporia of the activated and germinating spores were often decorated with mAb-nanoloops.
Keywords	Bacillus cereus Carcinomas Spores Large intestine Monocytes Neutrophils Respiratory tract cells Biological functions Anthracis Binding site
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Brookhaven National Lab., Upton, NY.; Brookhaven National Lab., Upton, NY. Materials and Chemical Sciences Div.; State Univ. of New York at Stony Brook. Dept. of Chemistry.; Department of Energy, Washington, DC.
Supplemental Notes	Prepared in cooperation with State Univ. of New York at Stony Brook. Dept. of Chemistry. and Brookhaven National Lab., Upton, NY. Materials and Chemical Sciences Div. Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200820
Contract Number	DE-AC02-98CH10886

Exosporium of B. Cereus Contains a Binding Site for gC1qR/p33: Implication in Spore Attachment and/or Entry.

Exosporium of B. Cereus Contains a Binding Site for gC1qR/p33: Implication in Spore Attachment and/or Entry.
DE2008924425

Publication Date	2008
Personal Author	Ghebrehiwet, B.; Wong, S. S.; Warren, J. B.; Titmus, M. A.
Page Count	19
Abstract	B. cereus, is a member of a genus of aerobic, gram-positive, spore-forming rod-like bacilli, which includes the deadly, B. anthracis. Preliminary experiments have shown that gC1qR binds to B. cereus spores that have been attached to microtiter plates. The present studies were therefore undertaken, to examine if cell surface gC1qR plays a role in B. cereus spore attachment and/or entry. Monolayers of human colon carcinoma (Caco-2) and lung cells were grown to confluency on 6 mm coverslips in shell vials with gentle swirling in a shaker incubator. Then, 2 (micro)l of a suspension of strain SB460 B.cereus spores (3x10(sup 8)/ml, in sterile water), were added and incubated (1-4 h; 36(sup 0) C) in the presence or absence of anti-gC1qR mAb-carbon nanoloops. Examination of these cells by EM revealed that: (1) When B. cereus endospores contacted the apical Caco-2 cell surface, or lung cells, gClqR was simultaneously detectable, indicating upregulation of the molecule. (2) In areas showing spore contact with the cell surface, gClqR expression was often adjacent to the spores in association with microvilli (Caco-2 cells) or cytoskeletal projections (lung cells). (3) Furthermore, the exosporia of the activated and germinating spores were often decorated with mAb-nanoloops.
Keywords	Bacillus cereus Carcinomas Spores Large intestine Monocytes Neutrophils Respiratory tract cells Biological functions Anthracis Binding site
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Brookhaven National Lab., Upton, NY.; Brookhaven National Lab., Upton, NY. Materials and Chemical Sciences Div.; State Univ. of New York at Stony Brook. Dept. of Chemistry.; Department of Energy, Washington, DC.
Supplemental Notes	Prepared in cooperation with State Univ. of New York at Stony Brook. Dept. of Chemistry. and Brookhaven National Lab., Upton, NY. Materials and Chemical Sciences Div. Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200820
Contract Number	DE-AC02-98CH10886