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Exploration of Simple Analytical Approaches for Rapid Detection of Pathogenic Bacteria.

DE2006861631

Publication Date	2006
Personal Author	Rahman, S.
Page Count	180
Abstract	Many of the current methods for pathogenic bacterial detection require long sample-preparation and analysis time, as well as complex instrumentation. This dissertation explores simple analytical approaches (e.g., flow cytometry and diffuse reflectance spectroscopy) that may be applied towards ideal requirements of a microbial detection system, through method and instrumentation development, and by the creation and characterization of immunosensing platforms. This dissertation is organized into six sections. In the general introduction section a literature review on several of the key aspects of this work is presented. First, different approaches for detection of pathogenic bacteria will be reviewed, with a comparison of the relative strengths and weaknesses of each approach. A general overview regarding diffuse reflectance spectroscopy is then presented. Next, the structure and function of self-assembled monolayers (SAMs) formed from organsulfur molecules at gold and micrometer and sub-micrometer patterning of biomolecules using SAMs will be discussed.
Keywords	Detection Bacteria Contamination Exploration Targets Quartz Reflection Sample preparation Pathogenic bacteria
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Iowa State Univ., Ames.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200618

Exploration of Simple Analytical Approaches for Rapid Detection of Pathogenic Bacteria.

Exploration of Simple Analytical Approaches for Rapid Detection of Pathogenic Bacteria.
DE2006861631

Publication Date	2006
Personal Author	Rahman, S.
Page Count	180
Abstract	Many of the current methods for pathogenic bacterial detection require long sample-preparation and analysis time, as well as complex instrumentation. This dissertation explores simple analytical approaches (e.g., flow cytometry and diffuse reflectance spectroscopy) that may be applied towards ideal requirements of a microbial detection system, through method and instrumentation development, and by the creation and characterization of immunosensing platforms. This dissertation is organized into six sections. In the general introduction section a literature review on several of the key aspects of this work is presented. First, different approaches for detection of pathogenic bacteria will be reviewed, with a comparison of the relative strengths and weaknesses of each approach. A general overview regarding diffuse reflectance spectroscopy is then presented. Next, the structure and function of self-assembled monolayers (SAMs) formed from organsulfur molecules at gold and micrometer and sub-micrometer patterning of biomolecules using SAMs will be discussed.
Keywords	Detection Bacteria Contamination Exploration Targets Quartz Reflection Sample preparation Pathogenic bacteria
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Iowa State Univ., Ames.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200618