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Investigation and Testing of Methods to Measure Changes in Microbial Populations Due to the Use of Oxygenates in Fuels Released to the Subsurface.

DE200515013325

Publication Date	2002
Personal Author	Happel, A.; Legler, T.; Kane, S.
Page Count	20
Abstract	Current bioremediation and risk-management practices are being adapted to the increasing possibility of encountering oxygenates (primarily methyl tert-butyl ether, MTBE, and ethanol) as co-contaminants at the approximately 400,000 leaking underground fuel tank (LUFT) sites nationwide. However, little is known about the effect of oxygenates on microbial populations and the resulting shifts in the microbial ecology and catabolic diversity that may result from the inclusion of oxygenates in reformulated gasoline. Among the possible effects are enrichment of oxygenate degrading bacteria in relation to BTEX (benzene, toluene, ethylbenzene and xylenes)- degrading bacteria, the fortuitous enrichment of bacteria that can degrade both oxygenates and BTEX' compounds, no effect on BTEX-degrading populations, or decreases in populations of certain bacteria as a result of toxicity. This study provided some preliminary information for the development of methods to determine the effect of oxygenates on the microbial community responsible for aerobic BTEX degradation.
Keywords	Oxygenates Microorganisms Methods investigations Methods testing Population changes Toxicity Co-contaminants Underground fuel tanks Subsurfaces Reformulated gasoline Degrading bacteria BTEX Catabolic diversity
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Lawrence Livermore National Lab., CA.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200524

Investigation and Testing of Methods to Measure Changes in Microbial Populations Due to the Use of Oxygenates in Fuels Released to the Subsurface.

Investigation and Testing of Methods to Measure Changes in Microbial Populations Due to the Use of Oxygenates in Fuels Released to the Subsurface.
DE200515013325

Publication Date	2002
Personal Author	Happel, A.; Legler, T.; Kane, S.
Page Count	20
Abstract	Current bioremediation and risk-management practices are being adapted to the increasing possibility of encountering oxygenates (primarily methyl tert-butyl ether, MTBE, and ethanol) as co-contaminants at the approximately 400,000 leaking underground fuel tank (LUFT) sites nationwide. However, little is known about the effect of oxygenates on microbial populations and the resulting shifts in the microbial ecology and catabolic diversity that may result from the inclusion of oxygenates in reformulated gasoline. Among the possible effects are enrichment of oxygenate degrading bacteria in relation to BTEX (benzene, toluene, ethylbenzene and xylenes)- degrading bacteria, the fortuitous enrichment of bacteria that can degrade both oxygenates and BTEX' compounds, no effect on BTEX-degrading populations, or decreases in populations of certain bacteria as a result of toxicity. This study provided some preliminary information for the development of methods to determine the effect of oxygenates on the microbial community responsible for aerobic BTEX degradation.
Keywords	Oxygenates Microorganisms Methods investigations Methods testing Population changes Toxicity Co-contaminants Underground fuel tanks Subsurfaces Reformulated gasoline Degrading bacteria BTEX Catabolic diversity
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Lawrence Livermore National Lab., CA.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200524