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Biodegradation of Chlorinated Solvents: Reactions near DNAPL and Enzyme Function.

DE2004833507

Publication Date	2002
Personal Author	McCarty, P. L.; Spormann, A. M.; Criddle, C. S.
Page Count	8
Abstract	The anaerobic biodegradation of chlorinated solvents is of great interest both for natural attenuation and for engineered remediation of these hazardous contaminants in groundwater. Compounds to be studied are carbon tetrachloride (CT) and the chlorinated ethenes, tetrachloroethene (PCE), trichloroethene (TCE) cis-1,2-dichloroethene (cDCE), and vinyl chloride (VC). The chlorinated solvents often are present as dense non-aqueous-phase liquids (DNAPLs), which are difficult to remove. Biodegradation of DNAPLs was previously thought not possible because of toxicity, but recent evidence indicates that under the right conditions, biodegradation is possible. Anaerobic biodegradation of DNAPLs is the major subject of this research. The specific objectives of this multi-investigator effort are: (1) Evaluate the potential for chlorinated solvent biodegradation near DNAPLs, (2) Provide a molecular understanding of the biological mechanisms involved, (3) Determine cellular components involved in carbon tetrachloride transformation by Pseudomonas stutzeri strain KC without chloroform formation.
Keywords	Water pollution control Chlorinated solvents Biodegradation Carbon tetrachloride Chloroform Enzymes Hazardous contaminants Pseudomonas Strainss Toxicity Transformations Vinyl chloride Ground water Remediation
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Stanford Univ., CA. Dept. of Civil and Environmental Engineering.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200512

Biodegradation of Chlorinated Solvents: Reactions near DNAPL and Enzyme Function.

Biodegradation of Chlorinated Solvents: Reactions near DNAPL and Enzyme Function.
DE2004833507

Publication Date	2002
Personal Author	McCarty, P. L.; Spormann, A. M.; Criddle, C. S.
Page Count	8
Abstract	The anaerobic biodegradation of chlorinated solvents is of great interest both for natural attenuation and for engineered remediation of these hazardous contaminants in groundwater. Compounds to be studied are carbon tetrachloride (CT) and the chlorinated ethenes, tetrachloroethene (PCE), trichloroethene (TCE) cis-1,2-dichloroethene (cDCE), and vinyl chloride (VC). The chlorinated solvents often are present as dense non-aqueous-phase liquids (DNAPLs), which are difficult to remove. Biodegradation of DNAPLs was previously thought not possible because of toxicity, but recent evidence indicates that under the right conditions, biodegradation is possible. Anaerobic biodegradation of DNAPLs is the major subject of this research. The specific objectives of this multi-investigator effort are: (1) Evaluate the potential for chlorinated solvent biodegradation near DNAPLs, (2) Provide a molecular understanding of the biological mechanisms involved, (3) Determine cellular components involved in carbon tetrachloride transformation by Pseudomonas stutzeri strain KC without chloroform formation.
Keywords	Water pollution control Chlorinated solvents Biodegradation Carbon tetrachloride Chloroform Enzymes Hazardous contaminants Pseudomonas Strainss Toxicity Transformations Vinyl chloride Ground water Remediation
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Stanford Univ., CA. Dept. of Civil and Environmental Engineering.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200512