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Low-Level Detection of a Bacillus Anthracis Simulant Using Love-Wave Biosensors on 36 degree YX LiTaO(sub 3).

DE2004820881

Publication Date	2003
Personal Author	Branch, D. W.; Brozik, S.
Page Count	38
Abstract	Crucial to low-level detection of biowarfare agents in aqueous environments is the mass sensitivity optimization of Love-wave acoustic sensors. The present work is an experimental study of 36(sup o) YX cut LiTaO(sub 3) based Love-wave devices for detection of pathogenic spores in aqueous conditions. Given that the detection limit (DL) of Love-wave based sensors is a strong function of the overlying waveguide, two waveguide materials have been investigated, which are polyimide and polystyrene. To determine the mass sensitivity of Love-wave sensor, bovine serum albumin (BSA) protein was injected into the Love-wave test cell while recording magnitude and phase shift across each sensor. Polyimide had the lowest mass detection limit with an estimated value of 1-2 ng/cm(sup 2), as compared to polystyrene where DL= 2.0 ng/cm(sup 2). Suitable chemistries were used to orient antibodies on the Love-wave sensor using adsorbed protein G. The thickness of each biofilm was measured using ellipsometry from which the surface concentrations were calculated. The monoclonal antibody BD8 with a high degree of selectivity for anthrax spores was used to capture the non-pathogenic simulant B. thuringiensis B8 spores. Bacillus Subtilis spores were used as a negative control to determine whether significant non-specific binding would occur. Spore aliquots were prepared using an optical counting method, which permitted removal of background particles for consistent sample preparation. This work demonstrates that Love-wave devices can be used to detect B. anthracis simulant below reported infectious levels.
Keywords	Biosensors Detection Acoustics Albumins Antibodies Bacillus subtilis Cattle Ellipsometry Optimization Phase shift Polystyrene Proteins Sample preparation Pathogenic spores Aqueous environments Waveguides Bacillus anthracis Biowarefare agents Love-wave acoustic sensors LiTaO3
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Sandia National Labs., Albuquerque, NM.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200412

Low-Level Detection of a Bacillus Anthracis Simulant Using Love-Wave Biosensors on 36 degree YX LiTaO(sub 3).

Low-Level Detection of a Bacillus Anthracis Simulant Using Love-Wave Biosensors on 36 degree YX LiTaO(sub 3).
DE2004820881

Publication Date	2003
Personal Author	Branch, D. W.; Brozik, S.
Page Count	38
Abstract	Crucial to low-level detection of biowarfare agents in aqueous environments is the mass sensitivity optimization of Love-wave acoustic sensors. The present work is an experimental study of 36(sup o) YX cut LiTaO(sub 3) based Love-wave devices for detection of pathogenic spores in aqueous conditions. Given that the detection limit (DL) of Love-wave based sensors is a strong function of the overlying waveguide, two waveguide materials have been investigated, which are polyimide and polystyrene. To determine the mass sensitivity of Love-wave sensor, bovine serum albumin (BSA) protein was injected into the Love-wave test cell while recording magnitude and phase shift across each sensor. Polyimide had the lowest mass detection limit with an estimated value of 1-2 ng/cm(sup 2), as compared to polystyrene where DL= 2.0 ng/cm(sup 2). Suitable chemistries were used to orient antibodies on the Love-wave sensor using adsorbed protein G. The thickness of each biofilm was measured using ellipsometry from which the surface concentrations were calculated. The monoclonal antibody BD8 with a high degree of selectivity for anthrax spores was used to capture the non-pathogenic simulant B. thuringiensis B8 spores. Bacillus Subtilis spores were used as a negative control to determine whether significant non-specific binding would occur. Spore aliquots were prepared using an optical counting method, which permitted removal of background particles for consistent sample preparation. This work demonstrates that Love-wave devices can be used to detect B. anthracis simulant below reported infectious levels.
Keywords	Biosensors Detection Acoustics Albumins Antibodies Bacillus subtilis Cattle Ellipsometry Optimization Phase shift Polystyrene Proteins Sample preparation Pathogenic spores Aqueous environments Waveguides Bacillus anthracis Biowarefare agents Love-wave acoustic sensors LiTaO3
Source Agency	Technical Information Center Oak Ridge Tennessee
Corporate Authors	Sandia National Labs., Albuquerque, NM.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	200412