National Technical Reports Library

The National Technical Information Service acquires, indexes, abstracts, and archives the largest collection of U.S. government-sponsored technical reports in existence. The NTRL offers online, free and open access to these authenticated government technical reports. Technical reports and documents in its repository may be available online for free either from the issuing federal agency, the U.S. Government Publishing Office’s Federal Digital System website, or through search engines.

Details

Non-Invasive Early Detection and Molecular Analysis of Low X-Ray Dose Effects in the Lens.

DE141136122

Publication Date	2013
Personal Author	Goldstein, L.
Page Count	8
Abstract	This is the Final Progress Report for DOE-funded research project DE- SC0002213 titled Non-invasive Early Detection and Molecular Analysis of Low X-ray Dose Effects in the Lens. The project focuses on the effects of low-linear energy transfer (LET) radiation on the ocular lens. The lens is an exquisitely radiosensitive tissue with a highly-ordered molecular structure that is amenable to non-invasive optical study from the periphery. These merits point to the lens as an ideal target for laser-based molecular biodosimetry (MBD). Following exposure to different types of ionizing radiations, the lens demonstrates molecular changes (e.g., oxidation, racemization, crosslinkage, truncation, aggregation, etc.) that impact the structure and function of the long-lived proteins in the cytosol of lens fiber cells. The vast majority of proteins in the lens comprise the highly-ordered crystalline. These highly conserved lens proteins are amongst the most concentrated and stable in the body. Once synthesized, the crystalline are retained in the fiber cell cytoplasm for life. Taken together, these properties point to the lens as an ideal system for quantitative in vivo MBD assessment using quasi-elastic light scattering (QLS) analysis. In this project, we deploy a purpose-designed non-invasive infrared laser QLS instrument as a quantitative tool for longitudinal assessment of pre-cataractous molecular changes in the lenses of living mice exposed to low-dose low-LET radiation compared to non-irradiated sham controls. We hypothesize that radiation exposure will induce dose-dependent changes in the molecular structure of matrix proteins in the lens. Mechanistic assays to ascertain radiation-induced molecular changes in the lens focus on protein aggregation and gene/protein expression patterns. We anticipate that this study will contribute to our understanding of early molecular changes associated with radiation-induced tissue pathology. This study also affords potential for translational development of molecular biodosimetry instrumentation to assess human exposure to mixed radiation fields.
Keywords	Molecular analysis Radiation doses Lenses Detection Energy transfer Eye(Anatomy) Light scattering Optical systems Proteins Radiation exposure Research project
Source Agency	Technical Information Center Oak Ridge Tennessee
NTIS Subject Category	57E - Clinical Medicine 57V - Radiobiology
Corporate Authors	Tufts Univ., Boston, MA. School of Medicine.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	201502
Contract Number	DE-SC0002213

Non-Invasive Early Detection and Molecular Analysis of Low X-Ray Dose Effects in the Lens.

Non-Invasive Early Detection and Molecular Analysis of Low X-Ray Dose Effects in the Lens.
DE141136122

Publication Date	2013
Personal Author	Goldstein, L.
Page Count	8
Abstract	This is the Final Progress Report for DOE-funded research project DE- SC0002213 titled Non-invasive Early Detection and Molecular Analysis of Low X-ray Dose Effects in the Lens. The project focuses on the effects of low-linear energy transfer (LET) radiation on the ocular lens. The lens is an exquisitely radiosensitive tissue with a highly-ordered molecular structure that is amenable to non-invasive optical study from the periphery. These merits point to the lens as an ideal target for laser-based molecular biodosimetry (MBD). Following exposure to different types of ionizing radiations, the lens demonstrates molecular changes (e.g., oxidation, racemization, crosslinkage, truncation, aggregation, etc.) that impact the structure and function of the long-lived proteins in the cytosol of lens fiber cells. The vast majority of proteins in the lens comprise the highly-ordered crystalline. These highly conserved lens proteins are amongst the most concentrated and stable in the body. Once synthesized, the crystalline are retained in the fiber cell cytoplasm for life. Taken together, these properties point to the lens as an ideal system for quantitative in vivo MBD assessment using quasi-elastic light scattering (QLS) analysis. In this project, we deploy a purpose-designed non-invasive infrared laser QLS instrument as a quantitative tool for longitudinal assessment of pre-cataractous molecular changes in the lenses of living mice exposed to low-dose low-LET radiation compared to non-irradiated sham controls. We hypothesize that radiation exposure will induce dose-dependent changes in the molecular structure of matrix proteins in the lens. Mechanistic assays to ascertain radiation-induced molecular changes in the lens focus on protein aggregation and gene/protein expression patterns. We anticipate that this study will contribute to our understanding of early molecular changes associated with radiation-induced tissue pathology. This study also affords potential for translational development of molecular biodosimetry instrumentation to assess human exposure to mixed radiation fields.
Keywords	Molecular analysis Radiation doses Lenses Detection Energy transfer Eye(Anatomy) Light scattering Optical systems Proteins Radiation exposure Research project
Source Agency	Technical Information Center Oak Ridge Tennessee
NTIS Subject Category	57E - Clinical Medicine 57V - Radiobiology
Corporate Authors	Tufts Univ., Boston, MA. School of Medicine.; Department of Energy, Washington, DC.
Supplemental Notes	Sponsored by Department of Energy, Washington, DC.
Document Type	Technical Report
NTIS Issue Number	201502
Contract Number	DE-SC0002213