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Molecular Anatomy of PFDA Hepatotoxicity as Studied by Two-Dimensional Electrophoresis.

ADA232958

Publication Date	1991
Personal Author	Witzmann, F. A.
Page Count	16
Abstract	Perfluoro-n-decanoic acid (PFDA) effects on protein expression in the rat liver were studied in rodents following in vivo exposure to PFDA levels above, below and at the LD-50. Two-dimensional whole-liver homogenate protein patterns were generated and compared to previous results. As before, numerous proteins were altered; some suppressed, some induced, but most were unaffected. In an effort to identify the altered proteins, further analysis off basic proteins by first-dimension NEPHGE revealed the induction of cytochrome P452 (lauric acid -oxidase) and enoyl-CoA hydratase.
Keywords	Accumulation Fatty acids Metabolism Molecules Proteins Rats Toxic agents Perfluoro-n-decanoic acid Hepatotoxicity Two-dimensional electrophoresis Cytochromes Liver enzymes
Source Agency	Air Force Non Paid ADAS
NTIS Subject Category	57Y - Toxicology 57F - Cytology, Genetics, & Molecular Biology
Corporate Authors	Indiana Univ.-Purdue Univ. at Indianapolis. Dept. of Biology.; Air Force Office of Scientific Research, Bolling AFB, DC.
Document Type	Technical Report
Title Note	Annual rept. 15 Dec 89-14 Dec 90.
NTIS Issue Number	199116

Molecular Anatomy of PFDA Hepatotoxicity as Studied by Two-Dimensional Electrophoresis.

Molecular Anatomy of PFDA Hepatotoxicity as Studied by Two-Dimensional Electrophoresis.
ADA232958

Publication Date	1991
Personal Author	Witzmann, F. A.
Page Count	16
Abstract	Perfluoro-n-decanoic acid (PFDA) effects on protein expression in the rat liver were studied in rodents following in vivo exposure to PFDA levels above, below and at the LD-50. Two-dimensional whole-liver homogenate protein patterns were generated and compared to previous results. As before, numerous proteins were altered; some suppressed, some induced, but most were unaffected. In an effort to identify the altered proteins, further analysis off basic proteins by first-dimension NEPHGE revealed the induction of cytochrome P452 (lauric acid -oxidase) and enoyl-CoA hydratase.
Keywords	Accumulation Fatty acids Metabolism Molecules Proteins Rats Toxic agents Perfluoro-n-decanoic acid Hepatotoxicity Two-dimensional electrophoresis Cytochromes Liver enzymes
Source Agency	Air Force Non Paid ADAS
NTIS Subject Category	57Y - Toxicology 57F - Cytology, Genetics, & Molecular Biology
Corporate Authors	Indiana Univ.-Purdue Univ. at Indianapolis. Dept. of Biology.; Air Force Office of Scientific Research, Bolling AFB, DC.
Document Type	Technical Report
Title Note	Annual rept. 15 Dec 89-14 Dec 90.
NTIS Issue Number	199116