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The National Technical Information Service acquires, indexes, abstracts, and archives the largest collection of U.S. government-sponsored technical reports in existence. The NTRL offers online, free and open access to these authenticated government technical reports. Technical reports and documents in its repository may be available online for free either from the issuing federal agency, the U.S. Government Publishing Office’s Federal Digital System website, or through search engines.

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Flow Cytometric Analysis of Hepatocytes from Normal, PFDA, and PH/DEN/PB-Treated Rats.

ADA223765

Publication Date	1989
Personal Author	Tarr, M. J.; Frazier, D. E.
Page Count	21
Abstract	The goal of this project was to provide preliminary data regarding the hepatotoxic and immunotoxic effects of perfluorodecanoic acid (PFDA). Flow cytometric evaluation of hepatocytes from PFDA-treated rats revealed an increase in size and granularity and a shift from tetraploidy towards diploidy, in rats treated with 50 mg/kg PFDA, as well as a suggestion of interference with lymphocyte blast transformation of splenocytes in these rats. These preliminary studies suggest that PFDA induces hepatocellular alterations which are detectable by flow cytometry, and induces both morphologic and possibly functional modulation of the immune system. Further studies are indicated to determine the significance of these findings. Keywords: Perfluorodecanoic acid(PFDA); Hepatocarcinogenesis; Preneoplastic lesions; Flow cytometry; Immunotoxicity. (jes)
Keywords	Liver Cells(Biology) Immunity Lesions Lymphocytes Modulation Rats Toxicology Perfluorodecanoic acid Flow cytometry Ploidies Blast transformation
Source Agency	Air Force Non Paid ADAS
NTIS Subject Category	57Y - Toxicology 57F - Cytology, Genetics, & Molecular Biology
Corporate Authors	Ohio State Univ. Research Foundation, Columbus.; Air Force Office of Scientific Research, Bolling AFB, DC.
Document Type	Technical Report
Title Note	Final rept.
NTIS Issue Number	199021

Flow Cytometric Analysis of Hepatocytes from Normal, PFDA, and PH/DEN/PB-Treated Rats.

Flow Cytometric Analysis of Hepatocytes from Normal, PFDA, and PH/DEN/PB-Treated Rats.
ADA223765

Publication Date	1989
Personal Author	Tarr, M. J.; Frazier, D. E.
Page Count	21
Abstract	The goal of this project was to provide preliminary data regarding the hepatotoxic and immunotoxic effects of perfluorodecanoic acid (PFDA). Flow cytometric evaluation of hepatocytes from PFDA-treated rats revealed an increase in size and granularity and a shift from tetraploidy towards diploidy, in rats treated with 50 mg/kg PFDA, as well as a suggestion of interference with lymphocyte blast transformation of splenocytes in these rats. These preliminary studies suggest that PFDA induces hepatocellular alterations which are detectable by flow cytometry, and induces both morphologic and possibly functional modulation of the immune system. Further studies are indicated to determine the significance of these findings. Keywords: Perfluorodecanoic acid(PFDA); Hepatocarcinogenesis; Preneoplastic lesions; Flow cytometry; Immunotoxicity. (jes)
Keywords	Liver Cells(Biology) Immunity Lesions Lymphocytes Modulation Rats Toxicology Perfluorodecanoic acid Flow cytometry Ploidies Blast transformation
Source Agency	Air Force Non Paid ADAS
NTIS Subject Category	57Y - Toxicology 57F - Cytology, Genetics, & Molecular Biology
Corporate Authors	Ohio State Univ. Research Foundation, Columbus.; Air Force Office of Scientific Research, Bolling AFB, DC.
Document Type	Technical Report
Title Note	Final rept.
NTIS Issue Number	199021