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Immune Reactivities of Antibodies against Glycolipids. II. Comparative Properties, using Liposomes, of Purified Antibodies against Mono-, Di- and Trihexosyl Ceramide Haptens.

ADA044379

Publication Date	1976
Personal Author	Alving, C. A.; Richards, R. L.
Page Count	9
Abstract	Specific antibodies against ceramid monohexosice (CMH), ceramide dihexoside (CDH) and ceramide trihexoside (CTH) were purified from antiserum or normal (unimmunized) rabbit serum by using liposomes as immunoadsorbents. Purified antibodies were compared with whole antiserum, or immunologically reactive normal rabbit serum, for specific activity against the immunizing antigen, and cross-reactivity with the other haptens. High levels of reactivity and reciprocal cross-reactivity were observed both with antisera and purified antibodies CMH and CDH. In contrast, although anti-CMH and anti-CDH sera both apparently cross-reacted' with CTH the purified antibodies in each case did not have any detectable cross-reactivity with CTH. Conversely, anti-CTH antiserum apparently cross-reacted with CDH, whereas purified antibodies did not. It was concluded that rabbit antiserum sometimes may contain naturally-occurring antibodies against a glycosphingolipid, and these may be eliminated by using purified non-cross-reacting antibodies. Naturally-occurring antibodies against CDH and CTH, but not abainst CMH were found in 10 normal rabbit sera. Purified naturally-occurring anti-CDH antibodies obtained from ofe of these rabbits did not cross-react with CTH, but purified anti-CTH from the same rabbit did cross-react with CDH.
Keywords	Glycolipids Immunity Antibodies Response(Biology) Reactivities Blood serum Rabbits Cytoplasm Membranes(Biology) Reprints Ceramide monohexoside Ceramide dihexoside Ceramide trihexoside Galactocerebroside Cytolipin Glycolipid hapten
Source Agency	Non Paid Reprints
NTIS Subject Category	57J - Immunology 57B - Biochemistry
Corporate Authors	Walter Reed Army Inst Of Research Washington D C
Supplemental Notes	See also AD-A044 374.
Document Type	Journal Article
NTIS Issue Number	197724

Immune Reactivities of Antibodies against Glycolipids. II. Comparative Properties, using Liposomes, of Purified Antibodies against Mono-, Di- and Trihexosyl Ceramide Haptens.

Immune Reactivities of Antibodies against Glycolipids. II. Comparative Properties, using Liposomes, of Purified Antibodies against Mono-, Di- and Trihexosyl Ceramide Haptens.
ADA044379

Publication Date	1976
Personal Author	Alving, C. A.; Richards, R. L.
Page Count	9
Abstract	Specific antibodies against ceramid monohexosice (CMH), ceramide dihexoside (CDH) and ceramide trihexoside (CTH) were purified from antiserum or normal (unimmunized) rabbit serum by using liposomes as immunoadsorbents. Purified antibodies were compared with whole antiserum, or immunologically reactive normal rabbit serum, for specific activity against the immunizing antigen, and cross-reactivity with the other haptens. High levels of reactivity and reciprocal cross-reactivity were observed both with antisera and purified antibodies CMH and CDH. In contrast, although anti-CMH and anti-CDH sera both apparently cross-reacted' with CTH the purified antibodies in each case did not have any detectable cross-reactivity with CTH. Conversely, anti-CTH antiserum apparently cross-reacted with CDH, whereas purified antibodies did not. It was concluded that rabbit antiserum sometimes may contain naturally-occurring antibodies against a glycosphingolipid, and these may be eliminated by using purified non-cross-reacting antibodies. Naturally-occurring antibodies against CDH and CTH, but not abainst CMH were found in 10 normal rabbit sera. Purified naturally-occurring anti-CDH antibodies obtained from ofe of these rabbits did not cross-react with CTH, but purified anti-CTH from the same rabbit did cross-react with CDH.
Keywords	Glycolipids Immunity Antibodies Response(Biology) Reactivities Blood serum Rabbits Cytoplasm Membranes(Biology) Reprints Ceramide monohexoside Ceramide dihexoside Ceramide trihexoside Galactocerebroside Cytolipin Glycolipid hapten
Source Agency	Non Paid Reprints
NTIS Subject Category	57J - Immunology 57B - Biochemistry
Corporate Authors	Walter Reed Army Inst Of Research Washington D C
Supplemental Notes	See also AD-A044 374.
Document Type	Journal Article
NTIS Issue Number	197724