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Assessing Pollinator Communities via Environmental DNA (eDNA) Metacommunity Assay.


AD1135346

Publication Date 2020
Personal Author Davis, M.; Harper, L. R.; Molano-Flores, B.; Benito, J.; Niemiller, M. L.
Page Count 72
Abstract Pollinators provide essential ecosystem services and are critical components of resilient ecosystems. Yet, pollinators are suffering staggering global declines leading to increased listing under the Endangered Species Act. This presents challenges for DoD installations. The technical objectives of this project were to: 1) develop and validate broad-spectrum pollinator eDNA metabarcoding primers, 2) test the efficacy of multiple capture methods to obtain Next Generation Sequencing-ready pollinator eDNA from flowers, and 3) validate eDNA sampling and microfluidic metabarcoding methods in controlled and field experiments with mixed plant/pollinator communities.
Keywords
  • Lepidoptera
  • Wildlife
  • Cells
  • Habitats
  • Spiders
  • Birds
  • Eutrophication
  • Ecology
  • Computational biology
  • Entomology
  • United states
  • Department of defense
  • Pests
  • Data analysis
  • Fish
  • Gel electrophoresis
Source Agency
  • Non Paid ADAS
NTIS Subject Category
  • 57F - Cytology, Genetics, & Molecular Biology
  • 57H - Ecology
Corporate Authors ILLINOIS NATURAL HISTORY SURVEY CHAMPAIGN IL; STRATEGIC ENVIRONMENTAL RESEARCH AND DEVELOPMENT PROGRAM ALEXANDRIA VA; Strategic Environmental Research Development Program Alexandria VA
Document Type Technical Report
Title Note Technical Report, Final Report.
NTIS Issue Number 202309
Contract Number
  • 19-P-0025
Assessing Pollinator Communities via Environmental DNA (eDNA) Metacommunity Assay.
Assessing Pollinator Communities via Environmental DNA (eDNA) Metacommunity Assay.
AD1135346

  • Lepidoptera
  • Wildlife
  • Cells
  • Habitats
  • Spiders
  • Birds
  • Eutrophication
  • Ecology
  • Computational biology
  • Entomology
  • United states
  • Department of defense
  • Pests
  • Data analysis
  • Fish
  • Gel electrophoresis
  • Non Paid ADAS
  • 57F - Cytology, Genetics, & Molecular Biology
  • 57H - Ecology
  • 19-P-0025
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