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Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1.

AD1094854

Publication Date	2020
Personal Author	Van Doremalen, N.; Bushmaker, T.; Morris, D. H.; Holbrook, M. G.; Gamble, A.; Williamson, B. N.; Tamin, A.; Harcourt, J. L.; Thornburg, N. J.; Gerber, S. I.; Lloyd-Smith, J. O.; De Wit, E.; Munster, V. J.
Page Count	3
Abstract	A novel human coronavirus that is now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (formerly called HCoV-19) emerged in Wuhan, China, in late 2019 and is now causing a pandemic.1 We analyzed the aerosol and surface stability of SARS-CoV-2 and compared it with SARS-CoV-1, the most closely related human coronavirus.2We evaluated the stability of SARS-CoV-2 and SARS-CoV-1 in aerosols and on various surfaces and estimated their decay rates using a Bayesian regression model (see the Methods section in the Supplementary Appendix, available with the full text of this letter at NEJM.org). SARS-CoV-2nCoV-WA1-2020 (MN985325.1) and SARS-CoV-1Tor2 (AY274119.3) were the strains used. Aerosols(<5 micron) containing SARS-CoV-2 (105.25 50%tissue-culture infectious dose [TCID50] per milliliter)or SARS-CoV-1 (106.75-7.00 TCID50 per milliliter)were generated with the use of a three-jet Collison nebulizer and fed into a Goldberg drum to create an aerosolized environment. The inoculum resulted in cycle-threshold values between20 and 22, similar to those observed in samples obtained from the upper and lower respiratory tract in humans.
Keywords	Coronavirus Infectious diseases Viruses Diseases and disorders Disease outbreaks Virus diseases Respiratory tract diseases Environment Pandemics Aerosols Surfaces Stainless steel Microorganisms Surface stability Decay rate Plastic Copper Cardboard Stability kinetics Half life
Source Agency	Non Paid ADAS
NTIS Subject Category	57E - Clinical Medicine 57K - Microbiology
Corporate Authors	NATIONAL INST OF ALLERGY AND INFECTIOUS DISEASES HAMILTON MT HAMILTON
Supplemental Notes	New England Journal of Medicine, 01 Jan 0001, 01 Jan 0001,
Document Type	Technical Report
Title Note	Journal Article - Open Access
NTIS Issue Number	202016

Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1.

Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1.
AD1094854

Publication Date	2020
Personal Author	Van Doremalen, N.; Bushmaker, T.; Morris, D. H.; Holbrook, M. G.; Gamble, A.; Williamson, B. N.; Tamin, A.; Harcourt, J. L.; Thornburg, N. J.; Gerber, S. I.; Lloyd-Smith, J. O.; De Wit, E.; Munster, V. J.
Page Count	3
Abstract	A novel human coronavirus that is now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (formerly called HCoV-19) emerged in Wuhan, China, in late 2019 and is now causing a pandemic.1 We analyzed the aerosol and surface stability of SARS-CoV-2 and compared it with SARS-CoV-1, the most closely related human coronavirus.2We evaluated the stability of SARS-CoV-2 and SARS-CoV-1 in aerosols and on various surfaces and estimated their decay rates using a Bayesian regression model (see the Methods section in the Supplementary Appendix, available with the full text of this letter at NEJM.org). SARS-CoV-2nCoV-WA1-2020 (MN985325.1) and SARS-CoV-1Tor2 (AY274119.3) were the strains used. Aerosols(<5 micron) containing SARS-CoV-2 (105.25 50%tissue-culture infectious dose [TCID50] per milliliter)or SARS-CoV-1 (106.75-7.00 TCID50 per milliliter)were generated with the use of a three-jet Collison nebulizer and fed into a Goldberg drum to create an aerosolized environment. The inoculum resulted in cycle-threshold values between20 and 22, similar to those observed in samples obtained from the upper and lower respiratory tract in humans.
Keywords	Coronavirus Infectious diseases Viruses Diseases and disorders Disease outbreaks Virus diseases Respiratory tract diseases Environment Pandemics Aerosols Surfaces Stainless steel Microorganisms Surface stability Decay rate Plastic Copper Cardboard Stability kinetics Half life
Source Agency	Non Paid ADAS
NTIS Subject Category	57E - Clinical Medicine 57K - Microbiology
Corporate Authors	NATIONAL INST OF ALLERGY AND INFECTIOUS DISEASES HAMILTON MT HAMILTON
Supplemental Notes	New England Journal of Medicine, 01 Jan 0001, 01 Jan 0001,
Document Type	Technical Report
Title Note	Journal Article - Open Access
NTIS Issue Number	202016